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Literature DB >> 23498741

Analysis of ciliary membrane protein dynamics using SNAP technology.

Abstract

The sensory functions of the primary cilium rely on receptors and other membrane proteins that are specifically sorted to the ciliary compartment, which is a subdomain of the plasma membrane. Defects in this process underlie a large number of human diseases, yet it is poorly understood. Thus, it is of great interest to understand the mechanisms by which the cell sorts and traffics proteins to the ciliary membrane. Here, we provide an overview of our method to study the sorting and trafficking of ciliary membrane proteins using SNAP technology. This technology enables pulse-chase analysis of the movement of proteins through the endomembrane system and onto the cilium.

Entities: Chemical Disease Gene Species

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Year: 2013 PMID： 23498741 PMCID： PMC3844868 DOI： 10.1016/B978-0-12-397945-2.00011-1

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

15 in total

1. A general method for the covalent labeling of fusion proteins with small molecules in vivo.

Authors: Antje Keppler; Susanne Gendreizig; Thomas Gronemeyer; Horst Pick; Horst Vogel; Kai Johnsson
Journal: Nat Biotechnol Date: 2002-12-09 Impact factor: 54.908

2. An ER retention signal explains differences in surface expression of NMDA and AMPA receptor subunits.

Authors: H Xia; Z D Hornby; R C Malenka
Journal: Neuropharmacology Date: 2001-11 Impact factor: 5.250

Review 3. Overview of structure and function of mammalian cilia.

Authors: Peter Satir; Søren Tvorup Christensen
Journal: Annu Rev Physiol Date: 2007 Impact factor: 19.318

4. Temperature-sensitive steps in the transport of secretory proteins through the Golgi complex in exocrine pancreatic cells.

Authors: J Saraste; G E Palade; M G Farquhar
Journal: Proc Natl Acad Sci U S A Date: 1986-09 Impact factor: 11.205

5. Vertebrate Smoothened functions at the primary cilium.

Authors: Kevin C Corbit; Pia Aanstad; Veena Singla; Andrew R Norman; Didier Y R Stainier; Jeremy F Reiter
Journal: Nature Date: 2005-08-31 Impact factor: 49.962

6. Human ADPKD primary cyst epithelial cells with a novel, single codon deletion in the PKD1 gene exhibit defective ciliary polycystin localization and loss of flow-induced Ca2+ signaling.

Authors: Chang Xu; Sandro Rossetti; Lianwei Jiang; Peter C Harris; Ursa Brown-Glaberman; Angela Wandinger-Ness; Robert Bacallao; Seth L Alper
Journal: Am J Physiol Renal Physiol Date: 2006-11-07

7. Polycystin-2 traffics to cilia independently of polycystin-1 by using an N-terminal RVxP motif.

Authors: Lin Geng; Dayne Okuhara; Zhiheng Yu; Xin Tian; Yiqiang Cai; Sekiya Shibazaki; Stefan Somlo
Journal: J Cell Sci Date: 2006-03-14 Impact factor: 5.285

8. Kinetic analysis of secretory protein traffic and characterization of golgi to plasma membrane transport intermediates in living cells.

Authors: K Hirschberg; C M Miller; J Ellenberg; J F Presley; E D Siggia; R D Phair; J Lippincott-Schwartz
Journal: J Cell Biol Date: 1998-12-14 Impact factor: 10.539

Analysis of ciliary membrane protein dynamics using SNAP technology.

1. A general method for the covalent labeling of fusion proteins with small molecules in vivo.

2. An ER retention signal explains differences in surface expression of NMDA and AMPA receptor subunits.

Review 3. Overview of structure and function of mammalian cilia.

4. Temperature-sensitive steps in the transport of secretory proteins through the Golgi complex in exocrine pancreatic cells.

5. Vertebrate Smoothened functions at the primary cilium.

6. Human ADPKD primary cyst epithelial cells with a novel, single codon deletion in the PKD1 gene exhibit defective ciliary polycystin localization and loss of flow-induced Ca2+ signaling.

7. Polycystin-2 traffics to cilia independently of polycystin-1 by using an N-terminal RVxP motif.

8. Kinetic analysis of secretory protein traffic and characterization of golgi to plasma membrane transport intermediates in living cells.

Review 9. The vertebrate primary cilium is a sensory organelle.

10. The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence.

1. Newly synthesized polycystin-1 takes different trafficking pathways to the apical and ciliary membranes.

2. BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.