| Literature DB >> 23494438 |
Pedro M Baptista1, Dipen Vyas, Emma Moran, Zhan Wang, Shay Soker.
Abstract
As a result of significant progress made in the last years in developing methods of whole organ decellularization techniques, organ bioengineering may now look more feasible than ever before. In this chapter, we describe in detail the necessary steps in human liver bioengineering. These include ferret liver decellularization by detergent perfusion, human liver progenitor and endothelial cell isolation, and finally, liver bioscaffold recellularization in a perfusion bioreactor.Entities:
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Year: 2013 PMID: 23494438 DOI: 10.1007/978-1-62703-363-3_24
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745