Literature DB >> 23494422

Xeno-free adaptation and culture of human pluripotent stem cells.

Tori Sampsell-Barron1.   

Abstract

Human pluripotent stem cells (hPSCs), including embryonic stem (ES) and induced pluripotent stem (iPS) cells, have been historically cultured in media containing xenogeneic animal components. As hPSC-derived cells and tissues are being developed for human therapies, the application of culture systems to reduce potential immunoreactivity and improve reproducibility becomes increasingly vital. Methods for directly adapting hPSCs to a commercially available culture system free of nonhuman proteins (xeno-free) are described in this chapter.

Entities:  

Mesh：

Substances：

Year:  2013        PMID： 23494422     DOI： 10.1007/978-1-62703-363-3_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

Review 1.  Methods of induced pluripotent stem cells for clinical application.

Authors:  Tomohisa Seki; Keiichi Fukuda
Journal:  World J Stem Cells       Date:  2015-01-26       Impact factor: 5.326

2.  Efficient differentiation of human embryonic stem cells to arterial and venous endothelial cells under feeder- and serum-free conditions.

Authors:  Gopu Sriram; Jia Yong Tan; Intekhab Islam; Abdul Jalil Rufaihah; Tong Cao
Journal:  Stem Cell Res Ther       Date:  2015-12-30       Impact factor: 6.832

3.  Generation of Functional Cardiomyocytes from Efficiently Generated Human iPSCs and a Novel Method of Measuring Contractility.

Authors:  Sheeja Rajasingh; Jayakumar Thangavel; Andras Czirok; Saheli Samanta; Katherine F Roby; Buddhadeb Dawn; Johnson Rajasingh
Journal:  PLoS One       Date:  2015-08-03       Impact factor: 3.240

4.  Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions.

Authors:  Yoshikazu Kishino; Tomohisa Seki; Jun Fujita; Shinsuke Yuasa; Shugo Tohyama; Akira Kunitomi; Ryota Tabei; Kazuaki Nakajima; Marina Okada; Akinori Hirano; Hideaki Kanazawa; Keiichi Fukuda
Journal:  PLoS One       Date:  2014-05-13       Impact factor: 3.240

5.  Development of a Xeno-Free Feeder-Layer System from Human Umbilical Cord Mesenchymal Stem Cells for Prolonged Expansion of Human Induced Pluripotent Stem Cells in Culture.

Authors:  Qing Zou; Mingjun Wu; Liwu Zhong; Zhaoxin Fan; Bo Zhang; Qiang Chen; Feng Ma
Journal:  PLoS One       Date:  2016-02-16       Impact factor: 3.240

6.  Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions.

Authors:  Yoshikazu Kishino; Tomohisa Seki; Shinsuke Yuasa; Jun Fujita; Keiichi Fukuda
Journal:  J Vis Exp       Date:  2015-11-11       Impact factor: 1.355
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.