AIM: To compare the results of two targeted techniques to an open-ended technique in periodontitis patients, differentiated on the basis of smoking habit. MATERIALS & METHODS: Thirty periodontitis patients (15 smokers and 15 non-smokers) provided subgingival plaque samples for 16S rRNA gene amplicon sequencing, culturing and quantitative polymerase chain reaction (qPCR). RESULTS: No differences were found in the composition of the subgingival microbiome between smokers and non-smokers with culture and qPCR. With pyrosequencing, operational taxonomic units (OTUs) classified to genera Fusobacterium, Prevotella and Selenomonas were more abundant in smokers, while OTUs belonging to the genera Peptococcus and Capnocytophaga were more abundant in non-smokers. Principal coordinate analysis identified two clusters; one was composed mainly of smokers (80%) and revealed significantly lower taxonomic diversity, higher attachment loss and higher proportion of the genera Fusobacterium, Paludibacter and Desulfobubus. CONCLUSION: In periodontitis, there is a difference in the composition of the subgingival microbiome between smokers and non-smokers, as revealed by pyrosequencing. This difference was not identified by the targeted techniques. Low taxonomic diversity was associated with higher disease severity, especially in smokers. This supports the hypothesis of the ecological microbial-host interaction in the severity of periodontal disease.
AIM: To compare the results of two targeted techniques to an open-ended technique in periodontitispatients, differentiated on the basis of smoking habit. MATERIALS & METHODS: Thirty periodontitispatients (15 smokers and 15 non-smokers) provided subgingival plaque samples for 16S rRNA gene amplicon sequencing, culturing and quantitative polymerase chain reaction (qPCR). RESULTS: No differences were found in the composition of the subgingival microbiome between smokers and non-smokers with culture and qPCR. With pyrosequencing, operational taxonomic units (OTUs) classified to genera Fusobacterium, Prevotella and Selenomonas were more abundant in smokers, while OTUs belonging to the genera Peptococcus and Capnocytophaga were more abundant in non-smokers. Principal coordinate analysis identified two clusters; one was composed mainly of smokers (80%) and revealed significantly lower taxonomic diversity, higher attachment loss and higher proportion of the genera Fusobacterium, Paludibacter and Desulfobubus. CONCLUSION: In periodontitis, there is a difference in the composition of the subgingival microbiome between smokers and non-smokers, as revealed by pyrosequencing. This difference was not identified by the targeted techniques. Low taxonomic diversity was associated with higher disease severity, especially in smokers. This supports the hypothesis of the ecological microbial-host interaction in the severity of periodontal disease.
Authors: Jo L Freudenheim; Robert J Genco; Michael J LaMonte; Amy E Millen; Kathleen M Hovey; Xiaodan Mai; Ngozi Nwizu; Christopher A Andrews; Jean Wactawski-Wende Journal: Cancer Epidemiol Biomarkers Prev Date: 2015-12-21 Impact factor: 4.254
Authors: Mariana E Kirst; Eric C Li; Barnett Alfant; Yueh-Yun Chi; Clay Walker; Ingvar Magnusson; Gary P Wang Journal: Appl Environ Microbiol Date: 2014-11-14 Impact factor: 4.792
Authors: Xiaodan Mai; Robert J Genco; Michael J LaMonte; Kathleen M Hovey; Jo L Freudenheim; Christopher A Andrews; Jean Wactawski-Wende Journal: J Periodontol Date: 2015-10-29 Impact factor: 6.993
Authors: R A M Exterkate; E Zaura; B W Brandt; M J Buijs; J E Koopman; W Crielaard; J M Ten Cate Journal: Clin Oral Investig Date: 2014-08-10 Impact factor: 3.573