OBJECTIVE: The aim of this study was to determine whether Staphylococcus aureus could develop a resistance to 405 nm light. BACKGROUND DATA: Research indicates that certain wavelengths and treatment parameters of light promote growth of bacteria; however, our earlier work indicated that blue light effectively inactivates growth of S. aureus in vitro. METHODS: S. aureus was tested because of its frequent isolation from skin infections and wounds. The organism was treated with 405 nm light from supraluminous diodes at a dose of 9 J/cm(2). RESULTS: As anticipated, blue light produced a significant (p≤0.05) inactivation of S. aureus growth. Subsequent applications of blue light to subcultured generations of S. aureus were increasingly effective through four stages (generations). Beginning with stage five, a decrease in effectiveness (resistance) was observed. CONCLUSIONS: Appropriate doses of 405-nm blue light inhibit the growth of S. aureus in vitro. This research does suggest that S. aureus may be capable of developing resistance to blue light irradiation.
OBJECTIVE: The aim of this study was to determine whether Staphylococcus aureus could develop a resistance to 405 nm light. BACKGROUND DATA: Research indicates that certain wavelengths and treatment parameters of light promote growth of bacteria; however, our earlier work indicated that blue light effectively inactivates growth of S. aureus in vitro. METHODS:S. aureus was tested because of its frequent isolation from skin infections and wounds. The organism was treated with 405 nm light from supraluminous diodes at a dose of 9 J/cm(2). RESULTS: As anticipated, blue light produced a significant (p≤0.05) inactivation of S. aureus growth. Subsequent applications of blue light to subcultured generations of S. aureus were increasingly effective through four stages (generations). Beginning with stage five, a decrease in effectiveness (resistance) was observed. CONCLUSIONS: Appropriate doses of 405-nm blue light inhibit the growth of S. aureus in vitro. This research does suggest that S. aureus may be capable of developing resistance to blue light irradiation.
Authors: Yucheng Wang; Ying Wang; Yuguang Wang; Clinton K Murray; Michael R Hamblin; David C Hooper; Tianhong Dai Journal: Drug Resist Updat Date: 2017-10-13 Impact factor: 18.500
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Authors: Fenella D Halstead; Joanne E Thwaite; Rebecca Burt; Thomas R Laws; Marina Raguse; Ralf Moeller; Mark A Webber; Beryl A Oppenheim Journal: Appl Environ Microbiol Date: 2016-06-13 Impact factor: 4.792
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