Role of the neutrophil-lymphocyte count ratio in the differential diagnosis between pulmonary tuberculosis and bacterial community-acquired pneumonia.

BACKGROUND: Differential diagnosis between pulmonary tuberculosis (TB) and bacterial community-acquired pneumonia (CAP) is often challenging. The neutrophil-lymphocyte count ratio (NLR), a convenient marker of inflammation, has been demonstrated to be a useful biomarker for predicting bacteremia. We investigated the usefulness of the NLR for discriminating pulmonary TB from bacterial CAP in an intermediate TB-burden country.
METHODS: We retrospectively analyzed the clinical and laboratory characteristics of 206 patients suspected of having pulmonary TB or bacterial CAP from January 2009 to February 2011. The diagnostic ability of the NLR for differential diagnosis was evaluated and compared with that of C-reactive protein.
RESULTS: Serum NLR levels were significantly lower in patients with pulmonary TB than in patients with bacterial CAP (3.67±2.12 vs. 14.64±9.72, P<0.001). A NLR <7 was an optimal cut-off value to discriminate patients with pulmonary TB from patients with bacterial CAP (sensitivity 91.1%, specificity 81.9%, positive predictive value 85.7%, negative predictive value 88.5%). The area under the curve for the NLR (0.95, 95% confidence interval [CI], 0.91-0.98) was significantly greater than that of C-reactive protein (0.83, 95% CI, 0.76-0.88; P=0.0015).
CONCLUSIONS: The NLR obtained at the initial diagnostic stage is a useful laboratory marker to discriminate patients with pulmonary TB from patients with bacterial CAP in an intermediate TB-burden country.

INTRODUCTION

Community-acquired pneumonia (CAP) is one of the most important infectious causes of death in Korea [1]. Early diagnosis and appropriate administration of antibiotics are essential for reducing pneumonia-related morbidity and mortality [2]. Many studies conducted in high-tuberculosis (TB) burden countries have shown that Mycobacterium tuberculosis is a frequent cause of CAP; however, it is frequently difficult to distinguish pulmonary TB from bacterial CAP during the initial diagnostic stage [3, 4]. The clinical and radiographic features of TB are often nonspecific, and the sensitivity of microscopic examination of expectorated sputum for acid-fast bacilli is as low as 50-60% [5].

Delays in diagnosing pulmonary TB can have a negative effect on patient morbidity and mortality. Furthermore, nosocomial transmission of pulmonary TB to other hospitalized patients and healthcare workers can occur. Recently, some studies have suggested that biomarkers such as C-reactive protein (CRP), procalcitonin, and soluble triggering receptor expressed on myeloid cells may play a role in discriminating pulmonary TB from bacterial CAP [6-8]. However, not all of these biomarkers are available in countries with a high TB burden.

The neutrophil-lymphocyte count ratio (NLR) is a readily calculable laboratory marker used to evaluate systemic inflammation. A recent study demonstrated the usefulness of lymphocytopenia for predicting bacteremia by combining conventional infection markers [9]. Additionally, de Jager et al. [10] demonstrated that lymphocytopenia and the NLR were superior to CRP level, and white blood cell (WBC) and neutrophil counts for predicting bacteremia in an emergency care unit.

In this study, we investigated the role of the NLR in discriminating patients with active pulmonary TB from those with bacterial CAP and compared the diagnostic ability of the NLR to that of CRP and WBC.

METHODS

1. Patients

Consecutive adult patients older than 18 yr who were suspected of having CAP from January 2009 to February 2011 were included in this retrospective cohort study. Suspected CAP patients were defined as those with recent-onset respiratory symptoms or signs, abnormal infiltrates on chest radiography, and not recently hospitalized.

Exclusion criteria included use of antibiotics for more than 24 hr at the time of enrollment, conditions known to affect total and differential WBC counts such as hematologic disorders, chronic inflammatory conditions, current steroid therapy, and/or history of steroid use within 3 months before admission, history of chemotherapy or radiotherapy within 4 weeks before enrollment, absence of WBC differential count or CRP data, or change to other diagnosis such as pulmonary embolism, pulmonary edema, or lung cancer during follow-up.

Demographic data were collected and the Pneumonia Patient Outcomes Research Team (PORT) score was calculated [11]. The institutional review board approved this study, and it was conducted in accordance with the Declaration of Helsinki.

2. Diagnosis of pulmonary TB or bacterial CAP

Patients were assigned to the pulmonary TB group when 1) M. tuberculosis was cultured from sputum or bronchial lavage fluid, or 2) the concentration of adenosine deaminase was ≥70 IU/dL according to lymphocyte-dominant exudative pleural effusion combined with a lung parenchymal lesion suggesting pulmonary TB. Patients were considered to have bacterial CAP when 1) clinical signs and new infiltration on chest radiograph were evident and completely resolved after treatment with the appropriate antibiotics, 2) sputum or lavage fluid cultures were M. tuberculosis-negative during clinical follow-up, and 3) viral pathogens were not detected.

For microbiological detection in patients with bacterial CAP, we performed sputum Gram stains, cultures, and blood cultures. Urinary antigen assays were also used to detect Legionella pneumophila and Streptococcus pneumoniae according to clinical indication. In some patients, the virus was isolated from nasopharyngeal secretion specimens using a real-time PCR platform to detect influenza virus A or B, adenovirus, human metapneumovirus, respiratory syncytial virus, rhinovirus, and parainfluenza virus.

3. Inflammatory markers

WBC differential count and CRP were determined before antibiotic treatment. Total WBC, neutrophil, and lymphocyte counts were determined using a Sysmex XE-2100 Hematology Analyzer (Sysmex Corp., Kobe, Japan). NLR was defined as absolute neutrophil count divided by absolute lymphocyte count. CRP levels were measured using an automated latex-enhanced turbidimetric immunoassay (Dimension; Dade Behring, Newark, NJ, USA; TBA-200FR; Toshiba, Tokyo, Japan) within 1 hour of sample collection. WBC differential count, NLR, and CRP level were compared between patients with pulmonary TB and patients with bacterial CAP. These inflammatory markers were also compared between patients with pulmonary TB and patients with mild severity pneumonia with a PORT pneumonia severity index (PSI) risk class I or II.

4. Statistical analysis

Continuous data are presented as the mean±standard deviation. Differences in continuous variables between groups were determined using Student's t-test or the Mann-Whitney U-test. Categorical data are presented as frequencies. Differences between categorical variables were analyzed using Pearson's chi-square test or Fisher's exact test. Correlation between NLR and CRP was analyzed using Spearman's rank correlation test. ROC curve analysis was performed to identify the most useful cut-off levels for NLR, CRP, WBC count, neutrophil count, and lymphocyte count to identify the greatest sum of sensitivity and specificity for distinguishing pulmonary TB from bacterial CAP. The ability of NLR, CRP, WBC count, neutrophil count, and lymphocyte count to distinguish pulmonary TB from bacterial CAP was compared using the area under the curve (AUC). A P value ≤0.05 was considered statistically significant. All statistical analyses were performed using SPSS 19.0 (SPSS Inc., Chicago, IL, USA).

RESULTS

We enrolled 206 patients in this study (Fig. 1), of which 112 (54.4%) had pulmonary TB and 94 (45.6%) had bacterial CAP. The median age of patients with pulmonary TB and bacterial CAP was 54 yr (range, 20-86 yr) and 70 yr (range, 18-86 yr), respectively. Baseline clinical characteristics are listed in Table 1.

Fig. 1

Patient flow chart.

Abbreviations: WBC, white blood cell; CRP, C-reactive protein; FU, follow up; CAP, community-acquired pneumonia; TB, tuberculosis; AFB, acid-fastbacilli.

Table 1

Baseline clinical characteristics of the study population

Values are presented as median (range) or number (percentage).

Abbreviation: CAP, community-acquired pneumonia.

Among the patients with pulmonary TB, 98 (87.5%) had M. tuberculosis culture-positive respiratory specimens. The remaining patients had pleural effusion and lung infiltration on radiography compatible with TB. Treatment failure occurred in one patient.

The causative pathogens were determined in 27 patients (28.7%) with bacterial CAP. Streptococcus pneumoniae was detected in 17 patients using urinary antigen tests, respiratory specimen culture, or blood culture. Other pathogens identified were Klebsiella pneumonia (N=4), Escherichia coli (N=2), methicillin-sensitive Staphylococcus aureus (N=1), methicillin-resistant S. aureus (N=1), Acinetobacter baumannii (N=1), and Stenotrophomonas maltophila (N=1). The mean PSI was 111.03. Seventy-four patients (78.7%) were categorized as greater than PSI risk class III. Twenty patients (21.3%) were regarded as having mild-severity pneumonia. In-hospital mortality for the CAP group was 12.8% (N=12).

2. Inflammation markers

Mean and median values of the NLR were 9.69±9.02 and 6.36, respectively. Total WBC and neutrophil count, NLR, and CRP were significantly lower in patients with pulmonary TB than in patients with bacterial CAP, whereas lymphocyte count was significantly higher in patients with pulmonary TB than in patients with bacterial CAP (Table 2).

Table 2

Baseline laboratory characteristics of the study population

Values are presented as mean±standard deviation.

Abbreviations: CAP, community-acquired pneumonia; WBC, white blood cell; NLR, neutrophil-lymphocyte count ratio; CRP, C-reactive protein.

We conducted a subgroup analysis of patients with mild pneumonia (N=20, PSI risk classes I and II) and patients with pulmonary TB to assess the diagnostic ability of the NLR. Total WBC count (7.19±2.48 vs. 12.59±5.41, P<0.001), neutrophil count (4.79±2.14 vs. 10.38±4.78, P<0.001), NLR (3.67±2.12 vs. 9.65±6.41, P<0.001), and CRP (6.57±7.31 vs. 17.48±10.76, P<0.001) were also significantly lower in patients with pulmonary TB than in patients with mild-severity pneumonia. However, lymphocyte count was not significantly different between groups.

The strongest correlation was noted between NLR and neutrophil count (r=0.832, P=0.01). A positive correlation was also detected between NLR and CRP (r=0.562, P=0.01) as well as between NLR and WBC count (r=0.738, P=0.01). A negative correlation was identified between NLR and lymphocyte count (r=-0.644, P=0.01).

3. Diagnostic accuracy for discriminating pulmonary TB from bacterial CAP

A NLR<7.0 was identified as the optimal cut-off value for discriminating patients with pulmonary TB from patients with bacterial CAP, yielding 91.1% sensitivity, 81.9% specificity, 85.7% positive predictive value, and 88.5% negative predictive value (Table 3). The cut-off value for CRP was less than 7.0 mg/dL. Using this cut-off value, CRP showed 67.5% sensitivity and 85.1% specificity, 79.4% positive predictive value, and 75.5% negative predictive value. The NLR AUC (AUC, 0.93; 95% confidence interval [CI], 0.88-0.96) was significantly greater than that of CRP (AUC, 0.83; 95% CI, 0.76-0.88; P=0.0015) (Fig. 2). The NLR AUC was also significantly greater than that of WBC count (AUC, 0.86; 95% CI, 0.81-0.91; P=0.0016), neutrophil count (AUC, 0.89; 95% CI, 0.84-0.93; P=0.0197), and lymphocyte count (AUC, 0.74; 95% CI, 0.68-0.80; P<0.0001). In patients with mild pneumonia, the NLR AUC (AUC, 0.87; 95% CI, 0.76-0.92) was significantly greater than that of lymphocyte count (AUC, 0.61; 95% CI, 0.52-0.69; P=0.0019). However, there was no significant difference in the AUC between NLR and CRP (AUC, 0.81; 95% CI, 0.72-0.88; P=0.5721), NLR and WBC count (AUC, 0.81; 95% CI, 0.73-0.87; P=0.3523), and NLR and neutrophil count (AUC, 0.86; 95% CI, 0.79-0.91; P=0.8466).

Table 3

Diagnostic validity of NLR, CRP, and WBC with differential counts in differentiating pulmonary TB from bacterial CAP

Values are presented as percentages.

Cut-off values for each parameter: NLR* <7.0; CRP† < 7.0 mg/dL; WBC‡ <10.100×109/L; neutrophil§ <7.740×109/L; lymphocyte∥ >1.001×109/L.

Abbreviations: TB, tuberculosis; CAP, community-acquired pneumonia; NLR, neutrophil-lymphocyte count ratio; CRP, C-reactive protein; WBC, white blood cell; PPV, positive predictive value; NPV, negative predictive value.

Fig. 2

ROC curves of C-reactive protein (CRP) and neutrophil-lymphocyte count ratio (NLR) for discriminating tuberculosis from bacterial community-acquired pneumonia. The area under the curve for NLR (AUC, 0.93; 95% confidence interval [CI], 0.88-0.96) was significantly greater than that for CRP (AUC, 0.83; 95% CI, 0.76-0.88) (P=0.0015).

DISCUSSION

In this study of consecutive patients recruited from clinical settings, the NLR was a useful laboratory marker for discriminating between patients with pulmonary TB and other bacterial CAP at the initial diagnostic stage for intermediate TB prevalence. The findings of this study included the following: 1) Serum NLR levels were significantly lower in patients with pulmonary TB than in patients with bacterial CAP; 2) A NLR<7 was the optimal cut-off value for discriminating patients with pulmonary TB from patients with bacterial CAP; 3) The diagnostic ability of the NLR was superior to that of CRP, WBC count, neutrophil count, and lymphocyte count for discriminating patients with pulmonary TB from patients with bacterial CAP. This is the first study to demonstrate the diagnostic ability of the NLR for differentiating between pulmonary TB and bacterial CAP.

Korea is an intermediate TB-burden country, with approximately 43,000 cases of pulmonary TB newly diagnosed in 2009 [12]. The frequency of M. tuberculosis identification in patients with suspected CAP is as high as 12% in Hong Kong [4] and 21% in Singapore [13]; the prevalence of TB in these countries is similar to that in Korea. Distinguishing pulmonary TB from CAP is important in public health; immediate isolation and early treatment with anti-TB agents is essential for patients with pulmonary TB. However, differentiating between pulmonary TB and CAP based only on history, physical examination, and radiological findings is frequently difficult. Therefore, a rapid and readily available test to distinguish pulmonary TB from CAP is necessary.

The physiological immune responses of circulating leukocytes to various stressful events are characterized by an increased neutrophil count and decreased lymphocyte count. An increase in total WBC and neutrophils is an inflammatory reaction, particularly when caused by a bacterial infection [14]. Lymphocytopenia has also been described as a diagnostic marker of bacterial infection [9]. Therefore, the NLR is thought to have stronger discriminative power for predicting bacteremia compared to discrimination based on neutrophilia or lymphocytopenia alone. Goodman et al. [15] initially suggested the usefulness of the NLR for diagnosing patients with suspected appendicitis. In their study, the NLR was a more sensitive parameter of bacterial infection than an increased WBC count. Zahorec et al. [14] carried out a prospective longitudinal observational study, in which they reported a correlation between clinical course severity and lymphocytopenia in patients with severe sepsis and septic shock in an oncologic intensive care unit. They proposed using the NLR as an additional marker of infection in the intensive care unit [14]. Recently, de Jager et al. [10] demonstrated that both lymphocytopenia and the NLR were better predictors of bacteremia than routine parameters such as CRP level, WBC count, and neutrophil count in an emergency care unit. Currently, the NLR has been garnering interest as a survival predictor in various clinical situations, ranging from oncological to cardiovascular diseases [16-19].

The NLR of pleural fluid has been evaluated as an adjunctive diagnostic tool in patients with TB pleurisy [20, 21]. CD4+ T lymphocytes and macrophages are essential in the pathogenesis of TB since cell-mediated immunity is involved. In contrast, neutrophilia is the primary cellular phenomenon during development of bacterial CAP. Therefore, the NLR of blood samples may be a good laboratory marker for discriminating patients with pulmonary TB from patients with bacterial CAP.

In this study, total WBC with differential count, CRP, and NLR were significantly different between the pulmonary TB group and the bacterial CAP group. The NLR was the most powerful discriminative marker, as evidenced by its high AUC. Neutrophil count was the second best discriminative marker, followed by WBC count, CRP, and lymphocyte count. Notably, easily evaluable complete blood count parameters such as total WBC and neutrophil counts also demonstrated considerable discriminative power, although less than that of the NLR. Moreover, their sensitivities, specificities, and accuracies were relatively high.

The NLR can be easily determined from a routine laboratory exam, and this simple ratio is available in most institutions. Furthermore, the NLR is an easier and less expensive method compared to other inflammatory biomarkers such as CRP, procalcitonin, and soluble triggering receptor expressed on myeloid cells, which recent studies have suggested as differential diagnostic markers [6-8].

However, there are several limitations in this study. First, it was retrospective, nonrandomized, and conducted in a single center; thus, it was subject to selection bias. Investigations involving a larger sample size and prospective analysis are necessary. Second, CAP-causing pathogens could only be identified in a minority of patients. However, CAP causative pathogens are not clearly defined [22, 23]. Third, other inflammatory markers such as procalcitonin were not included and compared with the NLR in the current analysis. However, procalcitonin is an expensive exam that is not available in all hospitals.

In conclusion, the current study showed that the NLR derived from a single blood sample during the initial diagnostic stage is a very useful laboratory marker for discriminating patients with pulmonary TB from patients with bacterial CAP. The diagnostic ability of the NLR is superior to that of CRP, and WBC, neutrophil, and lymphocyte counts. Therefore, the NLR should be incorporated into routine exams in patients with an ambiguous diagnosis between pulmonary TB and bacterial CAP.