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Literature DB >> 23479788

Barcoding of Giardia duodenalis isolates and derived lines from an established cryobank by a mutation scanning-based approach.

Matthew J Nolan¹, Aaron R Jex, Jacqui A Upcroft, Peter Upcroft, Robin B Gasser.

Abstract

We barcoded 25 in vitro isolates (representing 92 samples) of Giardia duodenalis from humans and other animals, which have been assembled by the Upcroft team at the Queensland Institute of Medical Research over a period of almost three decades. We used mutation scanning-coupled sequencing of loci in the triosephosphate isomerase, glutamate dehydrogenase and β-giardin genes, combined with phylogenetic analysis, to genetically characterise them. Specifically, the isolates (n514) of G. duodenalis from humans from Australia (AD113; BRIS/83/HEPU/106; BRIS/87/HEPU/713; BRIS/89/HEPU/1003; BRIS/92/HEPU/1541; BRIS/92/HEPU/1590; BRIS/92/HEPU/2443; BRIS/93/HEPU/1706), Malaysia (KL/92/IMR/1106) and Afghanistan (WB), a cat from Australia (BAC2), a sheep from Canada (OAS1) and a sulphur-crested cockatoo from Australia (BRIS/95/HEPU/2041) represented assemblage A (sub-assemblage AI-1, AI-2 or AII-2); isolates (n510) from humans from Australia (BRIS/91/HEPU/1279; BRIS/92/HEPU/2342; BRIS/92/HEPU/2348; BRIS/93/HEPU/1638; BRIS/93/HEPU/1653; BRIS/93/HEPU/1705; BRIS/93/HEPU/1718; BRIS/93/HEPU/1727), Papua New Guinea (BRIS/92/HEPU/1487) and Canada (H7) represented assemblage B (sub-assemblage BIV) and an isolate from cattle from Australia (BRIS/92/HEPU/1709) had a match to assemblage E. Isolate BRIS/90/HEPU/1229 from a human from Australia was shown to represent a mixed population of assemblages A and B. These barcoded isolates (including stocks and derived lines) now allow direct comparisons of experimental data among laboratories and represent a massive resource for transcriptomic, proteomic, metabolic and functional genomic studies using advanced molecular technologies.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Year: 2011 PMID： 23479788 PMCID： PMC3810208 DOI： 10.1002/elps.201100283

Source DB: PubMed Journal: Electrophoresis ISSN： 0173-0835 Impact factor: 3.535

108 in total

1. Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.

Authors: Robin B Gasser; Min Hu; Neil B Chilton; Bronwyn E Campbell; Aaron J Jex; Domenico Otranto; Claudia Cafarchia; Ian Beveridge; Xingquan Zhu
Journal: Nat Protoc Date: 2006 Impact factor: 13.491

Review 2. Giardia: overview and update.

Authors: Y R Ortega; R D Adam
Journal: Clin Infect Dis Date: 1997-09 Impact factor: 9.079

3. Quinacrine-resistant Giardia duodenalis.

Authors: J A Upcroft; R W Campbell; P Upcroft
Journal: Parasitology Date: 1996-03 Impact factor: 3.234

4. The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems.

Authors: Erica Lasek-Nesselquist; David Mark Welch; Mitchell L Sogin
Journal: Int J Parasitol Date: 2010-03-30 Impact factor: 3.981

5. Lethal Giardia from a wild-caught sulphur-crested cockatoo (Cacatua galerita) established in vitro chronically infects mice.

Authors: J A Upcroft; P A McDonnell; A N Gallagher; N Chen; P Upcroft
Journal: Parasitology Date: 1997-05 Impact factor: 3.234

6. Identification of Giardia species and Giardia duodenalis assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers.

Authors: Simone M Cacciò; Relja Beck; Andre Almeida; Anna Bajer; Edoardo Pozio
Journal: Parasitology Date: 2010-03-17 Impact factor: 3.234

4. An image-based Pathogen Box screen identifies new compounds with anti-Giardia activity and highlights the importance of assay choice in phenotypic drug discovery.

Authors: Snigdha Tiash; Jake Saunders; Christopher J S Hart; John H Ryan; Andrew G Riches; Tina S Skinner-Adams
Journal: Int J Parasitol Drugs Drug Resist Date: 2020-03-13 Impact factor: 4.077

4 in total

Barcoding of Giardia duodenalis isolates and derived lines from an established cryobank by a mutation scanning-based approach.

1. Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.

Review 2. Giardia: overview and update.

3. Quinacrine-resistant Giardia duodenalis.

4. The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems.

5. Lethal Giardia from a wild-caught sulphur-crested cockatoo (Cacatua galerita) established in vitro chronically infects mice.

6. Identification of Giardia species and Giardia duodenalis assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers.

7. Population genetics provides evidence for recombination in Giardia.

Review 8. Current trends in research into the waterborne parasite Giardia.

9. Identification of assemblage A Giardia in white-tailed deer.

10. Occurrence and molecular characterization of Cryptosporidium and Giardia in captive wild ruminants in Belgium.

1. A novel in vitro image-based assay identifies new drug leads for giardiasis.

Review 2. The controversies surrounding Giardia intestinalis assemblages A and B.

3. Data from a proteomic baseline study of Assemblage A in Giardia duodenalis.

4. An image-based Pathogen Box screen identifies new compounds with anti-Giardia activity and highlights the importance of assay choice in phenotypic drug discovery.