BACKGROUND: Early detection of renal cell carcinoma using serum/plasma biomarkers remains challenging. To validate clinical performance of potential candidate markers for kidney tumors, three-marker assay composed of nicotinamide N-methyltransferase (NNMT), L-plastin (LCP1), and nonmetastatic cells 1 protein (NM23A) was evaluated. METHODS: Patients with kidney cancer and control group were included in the clinical evaluation. Participants were divided into cohorts representing the training group of control group including healthy and benign tumors (n = 102) and patients with kidney cancer (n = 87) that were used to identify criteria for scoring. Then, we developed a three-marker assay that was validated with a cohort of test group samples (n = 100). A scoring method based on the cut-point of each of the three markers was used to evaluate the diagnostic performance of the marker combination. RESULTS: Plasma levels of NNMT, LCP1, and NM23A were highly elevated in patients with kidney cancer (P < 0.0001). In 289 blind sample tests with control subjects (n = 175) and patients with kidney cancer (n = 114), the diagnostic accuracy of NNMT alone and the three-marker assay was 0.913 and 0.932, respectively. When 90% specificity was defined, the sensitivity of NNMT and the three-marker assay was 71.9% and 95.7%, respectively. The predictive value of the three-marker assay was 87.2% (+PPV) and 97% (-PPV). CONCLUSIONS: The composite assay with NNMT, LCP1, and NM23A was a promising novel serum marker assay for the early detection of malignant kidney tumors covering subtypes of RCC with high diagnostic characteristics. IMPACT: NNMT/LCP1/NM23A triple markers could be a helpful screening assay to detect early RCC.
BACKGROUND: Early detection of renal cell carcinoma using serum/plasma biomarkers remains challenging. To validate clinical performance of potential candidate markers for kidney tumors, three-marker assay composed of nicotinamide N-methyltransferase (NNMT), L-plastin (LCP1), and nonmetastatic cells 1 protein (NM23A) was evaluated. METHODS:Patients with kidney cancer and control group were included in the clinical evaluation. Participants were divided into cohorts representing the training group of control group including healthy and benign tumors (n = 102) and patients with kidney cancer (n = 87) that were used to identify criteria for scoring. Then, we developed a three-marker assay that was validated with a cohort of test group samples (n = 100). A scoring method based on the cut-point of each of the three markers was used to evaluate the diagnostic performance of the marker combination. RESULTS: Plasma levels of NNMT, LCP1, and NM23A were highly elevated in patients with kidney cancer (P < 0.0001). In 289 blind sample tests with control subjects (n = 175) and patients with kidney cancer (n = 114), the diagnostic accuracy of NNMT alone and the three-marker assay was 0.913 and 0.932, respectively. When 90% specificity was defined, the sensitivity of NNMT and the three-marker assay was 71.9% and 95.7%, respectively. The predictive value of the three-marker assay was 87.2% (+PPV) and 97% (-PPV). CONCLUSIONS: The composite assay with NNMT, LCP1, and NM23A was a promising novel serum marker assay for the early detection of malignant kidney tumors covering subtypes of RCC with high diagnostic characteristics. IMPACT: NNMT/LCP1/NM23A triple markers could be a helpful screening assay to detect early RCC.
Authors: David L Masica; Marco Dal Molin; Christopher L Wolfgang; Tyler Tomita; Mohammad R Ostovaneh; Amanda Blackford; Robert A Moran; Joanna K Law; Thomas Barkley; Michael Goggins; Marcia Irene Canto; Meredith Pittman; James R Eshleman; Syed Z Ali; Elliot K Fishman; Ihab R Kamel; Siva P Raman; Atif Zaheer; Nita Ahuja; Martin A Makary; Matthew J Weiss; Kenzo Hirose; John L Cameron; Neda Rezaee; Jin He; Young Joon Ahn; Wenchuan Wu; Yuxuan Wang; Simeon Springer; Luis L Diaz; Nickolas Papadopoulos; Ralph H Hruban; Kenneth W Kinzler; Bert Vogelstein; Rachel Karchin; Anne Marie Lennon Journal: J Am Med Inform Assoc Date: 2016-06-21 Impact factor: 4.497
Authors: Darinka Todorova Petrova; Frank Christian Schultze; Gunnar Brandhorst; Klaus-Dieter Luchs; Christof Lenz; Henning Urlaub; Diana Rubel; Oliver Gross; Philip D Walson; Michael Oellerich Journal: Proteome Sci Date: 2014-12-10 Impact factor: 2.480