BACKGROUND: Pseudomonas aeruginosa cells are present as biofilms in the paranasal sinuses and the lungs of chronically infected cystic fibrosis (CF) patients. Since different inflammatory responses and selective antibiotic pressures are acting in the sinuses compared with the lungs, we compared the adaptive profiles of mucoid and non-mucoid isolates from the two locations. METHODS: We studied the genetic basis of phenotypic diversification and gene expression profiles in sequential lung and sinus P. aeruginosa isolates from four chronically infected CF patients, including pre- and post-lung transplantation isolates. RESULTS: The same phenotypes caused by similar mutations and similar gene expression profiles were found in mucoid and non-mucoid isolates from the paranasal sinuses and from the lungs before and after transplantation. CONCLUSION: Bilateral exchange of P. aeruginosa isolates between the paranasal sinuses and the lungs occurs in chronically infected patients and extensive sinus surgery before the lung transplantation might prevent infection of the new lung.
BACKGROUND:Pseudomonas aeruginosa cells are present as biofilms in the paranasal sinuses and the lungs of chronically infected cystic fibrosis (CF) patients. Since different inflammatory responses and selective antibiotic pressures are acting in the sinuses compared with the lungs, we compared the adaptive profiles of mucoid and non-mucoid isolates from the two locations. METHODS: We studied the genetic basis of phenotypic diversification and gene expression profiles in sequential lung and sinus P. aeruginosa isolates from four chronically infectedCFpatients, including pre- and post-lung transplantation isolates. RESULTS: The same phenotypes caused by similar mutations and similar gene expression profiles were found in mucoid and non-mucoid isolates from the paranasal sinuses and from the lungs before and after transplantation. CONCLUSION: Bilateral exchange of P. aeruginosa isolates between the paranasal sinuses and the lungs occurs in chronically infectedpatients and extensive sinus surgery before the lung transplantation might prevent infection of the new lung.
Authors: Siddhartha G Kapnadak; Gregory A Kicska; Kathleen J Ramos; Desiree A Marshall; Tamara Y Carroll; Sudhakar N Pipavath; Michael S Mulligan; Christopher H Goss; Moira L Aitken Journal: J Cyst Fibros Date: 2017-07-28 Impact factor: 5.482
Authors: M Kolpen; T Bjarnsholt; C Moser; C R Hansen; L F Rickelt; M Kühl; C Hempel; T Pressler; N Høiby; P Ø Jensen Journal: Clin Exp Immunol Date: 2014-07 Impact factor: 4.330
Authors: Renan Marrichi Mauch; Claudio Lucio Rossi; Marcos Tadeu Nolasco da Silva; Talita Bianchi Aiello; José Dirceu Ribeiro; Antônio Fernando Ribeiro; Niels Høiby; Carlos Emilio Levy Journal: Med Microbiol Immunol Date: 2019-01-31 Impact factor: 3.402
Authors: Kevin J Choi; Tracy Z Cheng; Adam L Honeybrook; Alice L Gray; Laurie D Snyder; Scott M Palmer; Ralph Abi Hachem; David W Jang Journal: Int Forum Allergy Rhinol Date: 2017-12-14 Impact factor: 3.858