| Literature DB >> 23475686 |
Mina Edwards1, Lyndsey Houseman, Ian R Phillips, Elizabeth A Shephard.
Abstract
Primary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital.Entities:
Mesh：
Substances：
Year: 2013 PMID： 23475686 DOI： 10.1007/978-1-62703-321-3_24
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745