| Literature DB >> 23475676 |
Qian Cheng1, F Peter Guengerich.
Abstract
Microtiter plate-based fluorescence assays allow rapid measurement of the catalytic activities of cytochrome P450 oxygenases (P450s). We describe a high-throughput fluorescence assay of P450 3A4, one of the key enzymes involved in xenobiotic metabolism. The assay involves the oxidative debenzylation of 7-hydroxy-4-trifluoromethyl coumarin, producing an increase in fluorescence.Entities:
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Year: 2013 PMID： 23475676 PMCID： PMC4024206 DOI： 10.1007/978-1-62703-321-3_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745