| Literature DB >> 23475663 |
Douglas S Auld1, Henrike Veith, James J Cali.
Abstract
The cytochrome P450 (CYP) family contains 57 enzymes in humans. The activity of CYPs against xenobiotics is a primary consideration in drug optimization efforts. Here we describe a series of bioluminescent assays that enable the rapid profiling of CYP activity against compound collections. The assays employ a coupled-enzyme format where firefly luciferase is used to measure CYP enzyme activity through metabolism of pro-luciferase substrates.Entities:
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Year: 2013 PMID： 23475663 DOI： 10.1007/978-1-62703-321-3_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745