| Literature DB >> 23475189 |
Cecilia Castro1, Jan Krumsiek, Nicolas J Lehrbach, Steven A Murfitt, Eric A Miska, Julian L Griffin.
Abstract
daf-2 is one of the most studied mutants in C. elegans: it contains a deletion in the gene orthologue of the insulin/insulin-like growth factor (IGF) receptor. Using high resolution (1)H NMR spectroscopy, metabolomics has helped to dissect the metabolic consequences of altered daf-2 signalling. Here, we present a detailed metabolomic analysis of daf-2, using NMR spectroscopy, gas chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) to integrate information from different pathways. We have then used Pearson and partial correlation analysis to build networks to explore the central role of daf-2 in regulating fatty acid and amino acid metabolism. The results show the tight regulation between these two parts of the metabolome.Entities:
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Year: 2013 PMID： 23475189 PMCID： PMC7002165 DOI： 10.1039/c3mb25539e
Source DB: PubMed Journal: Mol Biosyst ISSN： 1742-2051