| Literature DB >> 23467098 |
Kae Sato1, Reina Ishii, Naoki Sasaki, Kiichi Sato, Mats Nilsson.
Abstract
Padlock rolling circle amplification (RCA) is a powerful analytical method for ultrasensitive DNA detection. Although there are some advantages to bead-based RCA, a detailed study of the relationship between the bead material and the efficiency of bead-based RCA has not been reported. Here, we compared the reaction efficiencies of bead-based RCA performed on two types of bead material: agarose and polystyrene. Agarose was a more suitable material for on-bead RCA. The calibration curve showed linearity between 0.05 and 1 nM, and the limit of detection was 9 pM (9 amol) for Salmonella DNA determination.Entities:
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Year: 2013 PMID： 23467098 DOI： 10.1016/j.ab.2013.02.016
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365