OBJECTIVES: Characterize the phenotypic features of smooth muscle cells (SMCs) in the wall of human saccular intracranial aneurysms (sIAs). METHODS AND RESULTS: We investigated by means of immunohistochemistry the expression of the cytoskeletal differentiation markers α-smooth muscle actin (α-SMA), smooth muscle myosin heavy chains (SMMHCs), and smoothelin in 26 sIAs and 15 nonaneurysmal cerebral arteries. In addition, S100A4, a recently identified marker of dedifferentiated SMCs in atherosclerotic plaques, was also investigated. Six sIAs and 5 nonaneurysmal arteries were used for morphometric analysis. sIAs displayed a significant medial atrophy compared with nonaneurysmal cerebral arteries; moreover, sIA SMCs showed marked decrease of α-SMA and SMMHCs expression and disappearance of smoothelin. Unexpectedly, S100A4 was strongly up-regulated in media SMCs of sIAs. CONCLUSIONS: In sIAs, media SMCs acquire a dedifferentiated phenotype and show de novo expression of S100A4, characteristic features of atherosclerotic plaque SMCs.
OBJECTIVES: Characterize the phenotypic features of smooth muscle cells (SMCs) in the wall of humansaccular intracranial aneurysms (sIAs). METHODS AND RESULTS: We investigated by means of immunohistochemistry the expression of the cytoskeletal differentiation markers α-smooth muscle actin (α-SMA), smooth muscle myosin heavy chains (SMMHCs), and smoothelin in 26 sIAs and 15 nonaneurysmal cerebral arteries. In addition, S100A4, a recently identified marker of dedifferentiated SMCs in atherosclerotic plaques, was also investigated. Six sIAs and 5 nonaneurysmal arteries were used for morphometric analysis. sIAs displayed a significant medial atrophy compared with nonaneurysmal cerebral arteries; moreover, sIA SMCs showed marked decrease of α-SMA and SMMHCs expression and disappearance of smoothelin. Unexpectedly, S100A4 was strongly up-regulated in media SMCs of sIAs. CONCLUSIONS: In sIAs, media SMCs acquire a dedifferentiated phenotype and show de novo expression of S100A4, characteristic features of atherosclerotic plaque SMCs.
Authors: A E Vanrossomme; O F Eker; J-P Thiran; G P Courbebaisse; K Zouaoui Boudjeltia Journal: AJNR Am J Neuroradiol Date: 2015-04-30 Impact factor: 3.825
Authors: Piyusha S Gade; Riikka Tulamo; Kee-Won Lee; Fernando Mut; Eliisa Ollikainen; Chih-Yuan Chuang; Bong Jae Chung; Mika Niemelä; Behnam Rezai Jahromi; Khaled Aziz; Alexander Yu; Fady T Charbel; Sepideh Amin-Hanjani; Juhana Frösen; Juan R Cebral; Anne M Robertson Journal: Arterioscler Thromb Vasc Biol Date: 2019-08-29 Impact factor: 8.311
Authors: Sandrine Morel; Mannekomba R Diagbouga; Nicolas Dupuy; Esther Sutter; Vincent Braunersreuther; Graziano Pelli; Marco Corniola; Renato Gondar; Max Jägersberg; Nathalie Isidor; Karl Schaller; Marie-Luce Bochaton-Piallat; Philippe Bijlenga; Brenda R Kwak Journal: J Neuropathol Exp Neurol Date: 2018-07-01 Impact factor: 3.685
Authors: Alexander G Bick; Hiroko Wakimoto; Kimberli J Kamer; Yasemin Sancak; Olga Goldberger; Anna Axelsson; Daniel M DeLaughter; Joshua M Gorham; Vamsi K Mootha; J G Seidman; Christine E Seidman Journal: Proc Natl Acad Sci U S A Date: 2017-10-09 Impact factor: 11.205