OBJECTIVES: The aim of the study was to determine whether the expression levels of specific circulating serum microRNAs (miRNAs) are associated with mandibular prognathism (MP). METHODS: Sixty subjects in the early permanent dentition stage and 23 in the mixed dentition stage with MP were identified. Sixty-eight normal control subjects in the early permanent dentition stage and 24 in the mixed dentition stage were recruited for comparison. According to the microarray-based expression profiling, four serum miRNAs (let-7i-3p, miR-595, miR-16-2-3p, and miR-367-5p) were validated. RESULTS: In the MP groups, let-7i-3p was significantly over-expressed in subjects in the early permanent (P < 0.0005) and mixed (P < 0.001) dentitions, and miR-595 was significantly under-expressed (P < 0.004) in subjects in the early permanent (P < 0.004) and mixed (P < 0.0005) dentitions, compared with normal control groups. Multiple logistic regression analysis and receiver operating characteristic curve analysis revealed that let-7i-3p and miR-595 were able to significantly discriminate MP subjects from normal controls. CONCLUSION: Let-7i-3p and miR-595 could be potential, non-invasive biomarkers for the accurate early detection and diagnosis of MP, which may result in improved clinical management.
OBJECTIVES: The aim of the study was to determine whether the expression levels of specific circulating serum microRNAs (miRNAs) are associated with mandibular prognathism (MP). METHODS: Sixty subjects in the early permanent dentition stage and 23 in the mixed dentition stage with MP were identified. Sixty-eight normal control subjects in the early permanent dentition stage and 24 in the mixed dentition stage were recruited for comparison. According to the microarray-based expression profiling, four serum miRNAs (let-7i-3p, miR-595, miR-16-2-3p, and miR-367-5p) were validated. RESULTS: In the MP groups, let-7i-3p was significantly over-expressed in subjects in the early permanent (P < 0.0005) and mixed (P < 0.001) dentitions, and miR-595 was significantly under-expressed (P < 0.004) in subjects in the early permanent (P < 0.004) and mixed (P < 0.0005) dentitions, compared with normal control groups. Multiple logistic regression analysis and receiver operating characteristic curve analysis revealed that let-7i-3p and miR-595 were able to significantly discriminate MP subjects from normal controls. CONCLUSION: Let-7i-3p and miR-595 could be potential, non-invasive biomarkers for the accurate early detection and diagnosis of MP, which may result in improved clinical management.
Authors: Mirte Scheper; Alessia Romagnolo; Zein Mersini Besharat; Anand M Iyer; Romina Moavero; Christoph Hertzberg; Bernhard Weschke; Kate Riney; Martha Feucht; Theresa Scholl; Borivoj Petrak; Alice Maulisova; Rima Nabbout; Anna C Jansen; Floor E Jansen; Lieven Lagae; Malgorzata Urbanska; Elisabetta Ferretti; Aleksandra Tempes; Magdalena Blazejczyk; Jacek Jaworski; David J Kwiatkowski; Sergiusz Jozwiak; Katarzyna Kotulska; Krzysztof Sadowski; Julita Borkowska; Paolo Curatolo; James D Mills; Eleonora Aronica Journal: Biomedicines Date: 2022-07-29
Authors: Jeanette M Metzger; Mary S Lopez; Jenna K Schmidt; Megan E Murphy; Raghu Vemuganti; Marina E Emborg Journal: Biomed Res Int Date: 2020-05-02 Impact factor: 3.411