Literature DB >> 23462951

A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules.

Xinggui Gu1, Guanxin Zhang, Zhuo Wang, Wenwen Liu, Le Xiao, Deqing Zhang.   

Abstract

Based on the consideration that compound 1 with a -PO3H2 group can be hydrolyzed into compound 3 which shows low solubility in aqueous solutions and thus aggregation can occur easily, a new fluorescence turn-on assay has been constructed for alkaline phosphatase (ALP) with compound 1. ALP at concentrations as low as 18 mU mL(-1) can be assayed with compound 1. Moreover, compound 1 has been successfully applied for ALP assay in living cells. Also, compound 1 is useful for screening inhibitors of ALP.

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Year:  2013        PMID： 23462951     DOI： 10.1039/c3an36784c

Source DB:  PubMed          Journal:  Analyst        ISSN： 0003-2654            Impact factor:   4.616


  6 in total

1.  A sensitive fluorescent probe for alkaline phosphatase and an activity assay based on the aggregation-induced emission effect.

Authors:  Wenjuan Zhang; Hanxiao Yang; Nan Li; Na Zhao
Journal:  RSC Adv       Date:  2018-04-20       Impact factor: 4.036

Review 2.  Interrogating Protein Phosphatases with Chemical Activity Probes.

Authors:  Garrett R Casey; Cliff I Stains
Journal:  Chemistry       Date:  2018-03-08       Impact factor: 5.236

3.  Extracellular 4'-phosphopantetheine is a source for intracellular coenzyme A synthesis.

Authors:  Balaji Srinivasan; Madina Baratashvili; Marianne van der Zwaag; Bart Kanon; Cristina Colombelli; Roald A Lambrechts; Onno Schaap; Ellen A Nollen; Ajda Podgoršek; Gregor Kosec; Hrvoje Petković; Susan Hayflick; Valeria Tiranti; Dirk-Jan Reijngoud; Nicola A Grzeschik; Ody C M Sibon
Journal:  Nat Chem Biol       Date:  2015-08-31       Impact factor: 15.040

4.  TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase.

Authors:  Akio Shiba; Emiko Kinoshita-Kikuta; Eiji Kinoshita; Tohru Koike
Journal:  Sensors (Basel)       Date:  2017-08-15       Impact factor: 3.576

5.  Manipulating and visualizing the dynamic aggregation-induced emission within a confined quartz nanopore.

Authors:  Yi-Lun Ying; Yuan-Jie Li; Ju Mei; Rui Gao; Yong-Xu Hu; Yi-Tao Long; He Tian
Journal:  Nat Commun       Date:  2018-09-07       Impact factor: 14.919

6.  A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum.

Authors:  Jeongmoo Lee; Seoyun Kim; Tae Hoon Kim; Seoung Ho Lee
Journal:  RSC Adv       Date:  2020-07-20       Impact factor: 4.036
  6 in total

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