| Literature DB >> 23462618 |
Abstract
The objective of this study was to compare the toxicological effects of different source-related ambient PM10 samples in regard to their chemical composition. In this context we investigated airborne PM from different sites in Aachen, Germany. For the toxicological investigation human alveolar epithelial cells (A549) and murine macrophages (RAW264.7) were exposed from 0 to 96 h to increasing PM concentrations (0-100 μg/ml) followed by analyses of cell viability, pro-inflammatory and oxidative stress responses. The chemical analysis of these particles indicated the presence of 21 elements, water-soluble ions and PAHs. The toxicological investigations of the PM10 samples demonstrated a concentration- and time-dependent decrease in cell viability and an increase in pro-inflammatory and oxidative stress markers.Entities:
Keywords: A549; Al; Aluminum; Arsenic; As; CAT; COPD; CYP; Ca; Cadmium; Calcium; Catalase; Cd; Chemical composition; Chromium; Chronic Obstructive Pulmonary Disease; Copper; Cr; Cu; Cytochrome; Cytotoxicity; ELISA; Enzyme Linked Immunosorbent Assay; FBS; Fe; Fetal Bovine Serum; GPx; GRc; GSH; GSSG; Glutathione; Glutathione Disulphide; Glutathione Peroxidase; Glutathione Reductase; IL; Inflammatory response; Interleukin; Iron; K; LVS; Lead; Low Volume Sampler; Magnesium; Manganese; Mg; Mn; Murine macrophage cells; NADP(+)/NADPH; NF-(K)B; Na; Ni; Nickel; Nicotinamide Adenine Dinucleotide Phosphate; Nuclear Factor-kappa B; Oxidative stress; PAH; PBS; PM; Particulate Matter; Particulate matter; Pb; Phosphate Buffered Saline; Polycyclic Aromatic Hydrocarbons; Potassium; RAW264.7; RNS; ROS; Reactive Nitrogen Species; Reactive Oxygen Species; SEM; SOD; Scanning electron microscopy; Sodium; Superoxide-Dismutase; TC; TNF-α; Total Carbon; Toxicological effects; Tumor Necrosis Factor-α; Type II human alveolar basal epithelial cells; UFP; Ultrafine Particulate Matter; V; VOC; Vanadium; Volatile Organic Compounds; Zinc; Zn
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Year: 2013 PMID： 23462618 DOI： 10.1016/j.envpol.2013.01.026
Source DB: PubMed Journal: Environ Pollut ISSN： 0269-7491 Impact factor: 8.071