OBJECT: To characterize the progression of injured tissue resulting from a permanent focal cerebral ischemia after the acute phase, Magnetic Resonance Imaging (MRI) monitoring was performed on adult male C57BL/6J mice in the subacute stages, and correlated to histological analyses. MATERIAL AND METHODS: Lesions were induced by electrocoagulation of the middle cerebral artery. Serial MRI measurements and weighted-images (T2, T1, T2* and Diffusion Tensor Imaging) were performed on a 9.4T scanner. Histological data (Cresyl-Violet staining and laminin-, Iba1- and GFAP-immunostainings) were obtained 1 and 2 weeks after the stroke. RESULTS: Two days after stroke, tissues assumed to correspond to the infarct core, were detected as a hyperintensity signal area in T2-weighted images. One week later, low-intensity signal areas appeared. Longitudinal MRI study showed that these areas remained present over the following week, and was mainly linked to a drop of the T2 relaxation time value in the corresponding tissues. Correlation with histological data and immuno-histochemistry showed that these areas corresponded to microglial cells. CONCLUSION: The present data provide, for the first time detailed MRI parameters of microglial cells dynamics, allowing its non-invasive monitoring during the chronic stages of a stroke. This could be particularly interesting in regards to emerging anti-inflammatory stroke therapies.
OBJECT: To characterize the progression of injured tissue resulting from a permanent focal cerebral ischemia after the acute phase, Magnetic Resonance Imaging (MRI) monitoring was performed on adult male C57BL/6J mice in the subacute stages, and correlated to histological analyses. MATERIAL AND METHODS: Lesions were induced by electrocoagulation of the middle cerebral artery. Serial MRI measurements and weighted-images (T2, T1, T2* and Diffusion Tensor Imaging) were performed on a 9.4T scanner. Histological data (Cresyl-Violet staining and laminin-, Iba1- and GFAP-immunostainings) were obtained 1 and 2 weeks after the stroke. RESULTS: Two days after stroke, tissues assumed to correspond to the infarct core, were detected as a hyperintensity signal area in T2-weighted images. One week later, low-intensity signal areas appeared. Longitudinal MRI study showed that these areas remained present over the following week, and was mainly linked to a drop of the T2 relaxation time value in the corresponding tissues. Correlation with histological data and immuno-histochemistry showed that these areas corresponded to microglial cells. CONCLUSION: The present data provide, for the first time detailed MRI parameters of microglial cells dynamics, allowing its non-invasive monitoring during the chronic stages of a stroke. This could be particularly interesting in regards to emerging anti-inflammatory stroke therapies.
Authors: Quan Jiang; Zheng Gang Zhang; Guang Liang Ding; Brian Silver; Li Zhang; He Meng; Mei Lu; Siamak Pourabdillah-Nejed-D; Lei Wang; Smita Savant-Bhonsale; Lian Li; Hassan Bagher-Ebadian; Jiani Hu; Ali S Arbab; Padmavathy Vanguri; James R Ewing; Karyn Alayne Ledbetter; Michael Chopp Journal: Neuroimage Date: 2006-07-24 Impact factor: 6.556
Authors: Lian Li; Quan Jiang; Li Zhang; Guangliang Ding; Zheng Gang Zhang; Qingjiang Li; James R Ewing; Mei Lu; Swayamprava Panda; Karyn A Ledbetter; Polly A Whitton; Michael Chopp Journal: Brain Res Date: 2006-12-26 Impact factor: 3.252
Authors: C Wiessner; P R Allegrini; K Rupalla; D Sauer; T Oltersdorf; A L McGregor; S Bischoff; B W Böttiger; H van der Putten Journal: Neurosci Lett Date: 1999-06-25 Impact factor: 3.046
Authors: Philip A Barber; Lisa Hoyte; David Kirk; Tad Foniok; Alastair Buchan; Ursula Tuor Journal: Neurosci Lett Date: 2005-11-04 Impact factor: 3.046
Authors: R M Dijkhuizen; S Knollema; H B van der Worp; G J Ter Horst; D J De Wildt; J W Berkelbach van der Sprenkel; K A Tulleken; K Nicolay Journal: Stroke Date: 1998-03 Impact factor: 7.914