Bhargavaea indica sp. nov., a member of the phylum Firmicutes, isolated from Arabian Sea sediment.

A Gram-positive, aerobic, coccoid-rod shaped, non-motile, catalase- and oxidase-positive bacterium, designated strain KJW98(T), was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic, non-endospore-forming and grew with 0-8.5% (w/v) NaCl, at 15-48°C and at pH 6.5-9.0, with optimum growth with 0.5% (w/v) NaCl, at 42°C and at pH 7.0-8.0. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain KJW98(T) forms a lineage within the genus Bhargavaea. The G+C content of the genomic DNA was 55 mol%. The DNA-DNA relatedness values of strain KJW98(T) with B. beijingensis DSM 19037(T), B. cecembensis LMG 24411(T) and B. ginsengi DSM 19038(T) were 43.2, 39 and 26.5%, respectively. The major fatty acids were anteiso-C15:0 (37.7%), iso-C15:0 (19.7%), anteiso-C17:0 (17.0%) and iso-C16:0 (11.1%). The predominant menaquinone was MK-8 and the cell-wall peptidoglycan was of A4α type with L-lysine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The phenotypic, genotypic and DNA-DNA relatedness data indicate that strain KJW98(T) should be distinguished from the members of the genus Bhargavaea, for which the name Bhargavaea indica sp. nov. is proposed with the type strain KJW98(T) (=KCTC 13583(T) =LMG 25219(T)).