OBJECTIVE: To study the intestinal dynamics characteristics of puerarin and other active ingredients in Gegenqinlian decoction. METHODS: Caco-2 cell monolayer model was used to research the transport of puerarin and baicalin, berberine hydrochloride, liquiritin when they were used in different compatibilities, meanwhile a ultra performance liquid chromato-graphic method was also developed. RESULTS: The concentration of puerarin, haicalin, berberine hydrochloride, liqpiritin in cell culture medium was measured and the apparent permeahility coefficients (Papp) as well as percent absorption (%) from apical side to basolateral side were calculated. The absorption characteristics of the four components were different when they were used in different compatibilities. Furthermore the Papp sequence of puerarin in specific groups were puerarin-baicalin-berberine-liquiritin group > puerarin-baicalin group > puerarin-berberine > puerarin-liquiritin group > single puerarin (P < 0. 05). CONCLUSION: The intestinal absorption dynamics process of the active ingredients in Gegenqinlian decoction changes as the change of prescription compatibilities. And the intestinal absorption characteristics of puerarin is enhanced by the use of other components.
OBJECTIVE: To study the intestinal dynamics characteristics of puerarin and other active ingredients in Gegenqinlian decoction. METHODS: Caco-2 cell monolayer model was used to research the transport of puerarin and baicalin, berberine hydrochloride, liquiritin when they were used in different compatibilities, meanwhile a ultra performance liquid chromato-graphic method was also developed. RESULTS: The concentration of puerarin, haicalin, berberine hydrochloride, liqpiritin in cell culture medium was measured and the apparent permeahility coefficients (Papp) as well as percent absorption (%) from apical side to basolateral side were calculated. The absorption characteristics of the four components were different when they were used in different compatibilities. Furthermore the Papp sequence of puerarin in specific groups were puerarin-baicalin-berberine-liquiritin group > puerarin-baicalin group > puerarin-berberine > puerarin-liquiritin group > single puerarin (P < 0. 05). CONCLUSION: The intestinal absorption dynamics process of the active ingredients in Gegenqinlian decoction changes as the change of prescription compatibilities. And the intestinal absorption characteristics of puerarin is enhanced by the use of other components.