OBJECTIVE: Using papain to prepare polypeptide from Eupolyphaga sinensis, then study the immune function of polypeptide from Eupolyphaga sinensis in vivo. METHODS: Used hydrolysis degree as index, pH value, enzyme dosage, thermometer reaction time were optimized. Studied the influence of polyeptide on the mice immune functions through mice immune organs index, phagocytic function and the level of IL-2. RESULTS: The optimum enzymolysis condition was as follows: pH 8.0, enzyme 1%, temperature 55 degrees C, reaction time 4. 5 h. In vivo test of mice demonstraed that, Eupolyphaga sinensis could elevate index of thymus and spleen, enhance the phagocytic function of macrophage and promote the level of IL-2 in serum. CONCLUSION: Eupolyphaga sinensis has immunoregulatory effect.
OBJECTIVE: Using papain to prepare polypeptide from Eupolyphaga sinensis, then study the immune function of polypeptide from Eupolyphaga sinensis in vivo. METHODS: Used hydrolysis degree as index, pH value, enzyme dosage, thermometer reaction time were optimized. Studied the influence of polyeptide on the mice immune functions through mice immune organs index, phagocytic function and the level of IL-2. RESULTS: The optimum enzymolysis condition was as follows: pH 8.0, enzyme 1%, temperature 55 degrees C, reaction time 4. 5 h. In vivo test of mice demonstraed that, Eupolyphaga sinensis could elevate index of thymus and spleen, enhance the phagocytic function of macrophage and promote the level of IL-2 in serum. CONCLUSION:Eupolyphaga sinensis has immunoregulatory effect.