J C Vendrig1, L E Coffeng, J Fink-Gremmels. 1. Faculty of Veterinary Medicine, Veterinary Pharmacology, Pharmacotherapy and Toxicology, Utrecht University, The Netherlands. j.c.vendrig@uu.nl
Abstract
REASONS FOR PERFORMING STUDY: Increasing evidence suggests that reactions to lipopolysaccharide (LPS), particularly in the gut, can be partly or completely mitigated by colostrum- and milk-derived oligosaccharides. Confirmation of this hypothesis could lead to the development of new therapeutic concepts. OBJECTIVES: To demonstrate the influence of equine colostral carbohydrates on the inflammatory response in an in vitro model with equine peripheral blood mononuclear cells (PBMCs). METHODS: Carbohydrates were extracted from mare colostrum, and then evaluated for their influence on LPS-induced inflammatory responses in PBMCs isolated from the same mares, mRNA expression of tumour necrosis factor-alpha, interleukin-6 and interleukin-10 was measured as well as the protein levels of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10). RESULTS: Equine colostral carbohydrates significantly reduced LPS-induced TNF-alpha protein at both times measured and significantly reduced LPS-induced TNF-alpha, IL-6 and IL-10 mRNA expression by PBMCs. Moreover, cell viability significantly increased in the presence of high concentrations of colostral carbohydrates. CONCLUSIONS: Carbohydrates derived from equine colostrum reduce LPS-induced inflammatory responses of equine PBMCs. POTENTIAL RELEVANCE: Colostrum and milk-derived carbohydrates are promising candidates for new concepts in preventive and regenerative medicine.
REASONS FOR PERFORMING STUDY: Increasing evidence suggests that reactions to lipopolysaccharide (LPS), particularly in the gut, can be partly or completely mitigated by colostrum- and milk-derived oligosaccharides. Confirmation of this hypothesis could lead to the development of new therapeutic concepts. OBJECTIVES: To demonstrate the influence of equine colostral carbohydrates on the inflammatory response in an in vitro model with equine peripheral blood mononuclear cells (PBMCs). METHODS:Carbohydrates were extracted from mare colostrum, and then evaluated for their influence on LPS-induced inflammatory responses in PBMCs isolated from the same mares, mRNA expression of tumour necrosis factor-alpha, interleukin-6 and interleukin-10 was measured as well as the protein levels of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10). RESULTS:Equine colostral carbohydrates significantly reduced LPS-induced TNF-alpha protein at both times measured and significantly reduced LPS-induced TNF-alpha, IL-6 and IL-10 mRNA expression by PBMCs. Moreover, cell viability significantly increased in the presence of high concentrations of colostral carbohydrates. CONCLUSIONS:Carbohydrates derived from equine colostrum reduce LPS-induced inflammatory responses of equine PBMCs. POTENTIAL RELEVANCE: Colostrum and milk-derived carbohydrates are promising candidates for new concepts in preventive and regenerative medicine.