PURPOSE: The aims of this work were to (a) develop an approach for ex vivo MR volumetry of human brain hemispheres that does not contaminate the results of histopathological examination, (b) longitudinally assess regional brain volumes postmortem, and (c) investigate the relationship between MR volumetric measurements performed in vivo and ex vivo. METHODS: An approach for ex vivo MR volumetry of human brain hemispheres was developed. Five hemispheres from elderly subjects were imaged ex vivo longitudinally. All datasets were segmented. The longitudinal behavior of volumes measured ex vivo was assessed. The relationship between in vivo and ex vivo volumetric measurements was investigated in seven elderly subjects imaged both antemortem and postmortem. RESULTS: This approach for ex vivo MR volumetry did not contaminate the results of histopathological examination. For a period of 6 months postmortem, within-subject volume variation across time points was substantially smaller than intersubject volume variation. A close linear correspondence was detected between in vivo and ex vivo volumetric measurements. CONCLUSION: Regional brain volumes measured with this approach for ex vivo MR volumetry remain relatively unchanged for a period of 6 months postmortem. Furthermore, the linear relationship between in vivo and ex vivo MR volumetric measurements suggests that this approach captures information linked to antemortem macrostructural brain characteristics.
PURPOSE: The aims of this work were to (a) develop an approach for ex vivo MR volumetry of human brain hemispheres that does not contaminate the results of histopathological examination, (b) longitudinally assess regional brain volumes postmortem, and (c) investigate the relationship between MR volumetric measurements performed in vivo and ex vivo. METHODS: An approach for ex vivo MR volumetry of human brain hemispheres was developed. Five hemispheres from elderly subjects were imaged ex vivo longitudinally. All datasets were segmented. The longitudinal behavior of volumes measured ex vivo was assessed. The relationship between in vivo and ex vivo volumetric measurements was investigated in seven elderly subjects imaged both antemortem and postmortem. RESULTS: This approach for ex vivo MR volumetry did not contaminate the results of histopathological examination. For a period of 6 months postmortem, within-subject volume variation across time points was substantially smaller than intersubject volume variation. A close linear correspondence was detected between in vivo and ex vivo volumetric measurements. CONCLUSION: Regional brain volumes measured with this approach for ex vivo MR volumetry remain relatively unchanged for a period of 6 months postmortem. Furthermore, the linear relationship between in vivo and ex vivo MR volumetric measurements suggests that this approach captures information linked to antemortem macrostructural brain characteristics.
Authors: Bruce Fischl; David H Salat; Evelina Busa; Marilyn Albert; Megan Dieterich; Christian Haselgrove; Andre van der Kouwe; Ron Killiany; David Kennedy; Shuna Klaveness; Albert Montillo; Nikos Makris; Bruce Rosen; Anders M Dale Journal: Neuron Date: 2002-01-31 Impact factor: 17.173
Authors: Ioannis S Gousias; Daniel Rueckert; Rolf A Heckemann; Leigh E Dyet; James P Boardman; A David Edwards; Alexander Hammers Journal: Neuroimage Date: 2007-12-03 Impact factor: 6.556
Authors: Claus Svarer; Karine Madsen; Steen G Hasselbalch; Lars H Pinborg; Steven Haugbøl; Vibe G Frøkjaer; Søren Holm; Olaf B Paulson; Gitte M Knudsen Journal: Neuroimage Date: 2004-12-09 Impact factor: 6.556
Authors: Rajendra A Morey; Christopher M Petty; Yuan Xu; Jasmeet Pannu Hayes; H Ryan Wagner; Darrell V Lewis; Kevin S LaBar; Martin Styner; Gregory McCarthy Journal: Neuroimage Date: 2008-12-30 Impact factor: 6.556
Authors: Nazanin Makkinejad; Julie A Schneider; Junxiao Yu; Sue E Leurgans; Aikaterini Kotrotsou; Arnold M Evia; David A Bennett; Konstantinos Arfanakis Journal: Neurobiol Aging Date: 2019-01-31 Impact factor: 4.673
Authors: Aikaterini Kotrotsou; Julie A Schneider; David A Bennett; Sue E Leurgans; Robert J Dawe; Patricia A Boyle; Tom Golak; Konstantinos Arfanakis Journal: Neurobiol Aging Date: 2015-06-30 Impact factor: 4.673
Authors: David A Bennett; Aron S Buchman; Patricia A Boyle; Lisa L Barnes; Robert S Wilson; Julie A Schneider Journal: J Alzheimers Dis Date: 2018 Impact factor: 4.472
Authors: Aron S Buchman; Robert J Dawe; Lei Yu; Andrew Lim; Robert S Wilson; Julie A Schneider; David A Bennett Journal: Neurology Date: 2018-04-25 Impact factor: 9.910
Authors: Davis C Woodworth; Hannah L Nguyen; Zainab Khan; Claudia H Kawas; María M Corrada; S Ahmad Sajjadi Journal: Alzheimers Dement Date: 2020-12-07 Impact factor: 21.566
Authors: Lei Yu; Patricia A Boyle; Robert J Dawe; David A Bennett; Konstantinos Arfanakis; Julie A Schneider Journal: Neurology Date: 2019-11-22 Impact factor: 9.910