Literature DB >> 23436374

Mammalian two-hybrid assays for studies of interaction of p300 with transcription factors.

Daniela B Mendonça1, Gustavo Mendonça, Lyndon F Cooper.   

Abstract

The two-hybrid system is a powerful genetic assay that allows the interaction between two proteins to be detected in vivo. It was originally described in 1989 and since then it has been one of the main techniques used to identify interactions between proteins from different cellular organisms. Here we describe the methods to study the interaction of p300 with other transcription factors, specifically between p300 and two transcription factors related to hypoxia and inflammation, HIF-1α and NF-κB-p65, respectively.

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Year:  2013        PMID： 23436374      PMCID： PMC4502915          DOI： 10.1007/978-1-62703-284-1_26

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

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Authors:  D Ladant; G Karimova
Journal:  Res Microbiol       Date:  2000-11       Impact factor: 3.992

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Journal:  Methods       Date:  2000-01       Impact factor: 3.608

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Authors:  X H Feng; R Derynck
Journal:  Methods Mol Biol       Date:  2001

4.  Studying protein-protein interactions using a bacterial two-hybrid system.

Authors:  Simon L Dove
Journal:  Methods Mol Biol       Date:  2003

5.  Identification of protein interaction partners by the yeast two-hybrid system.

Authors:  Maria-Paz Garcia-Cuellar; Deniz Mederer; Robert K Slany
Journal:  Methods Mol Biol       Date:  2009

6.  A novel genetic system to detect protein-protein interactions.

Authors:  S Fields; O Song
Journal:  Nature       Date:  1989-07-20       Impact factor: 49.962

Review 7.  Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.

Authors:  Jae Woon Lee; Soo-Kyung Lee
Journal:  Methods Mol Biol       Date:  2004

8.  Yeast and mammalian two-hybrid systems for studying protein-protein interactions.

Authors:  Shu-ichi Matsuzawa; John C Reed
Journal:  Methods Mol Biol       Date:  2007

9.  Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.

Authors:  Runtao He; Xuguang Li
Journal:  Methods Mol Biol       Date:  2008
  9 in total
  3 in total

1.  Assays to Interrogate the Ability of Compounds to Inhibit the AF-2 or AF-1 Transactivation Domains of the Androgen Receptor.

Authors:  Ashley T Fancher; Yun Hua; Christopher J Strock; Paul A Johnston
Journal:  Assay Drug Dev Technol       Date:  2019-09-06       Impact factor: 1.738

2.  Detecting protein-protein interactions based on kinase-mediated growth induction of mammalian cells.

Authors:  Satoru Mabe; Teruyuki Nagamune; Masahiro Kawahara
Journal:  Sci Rep       Date:  2014-08-19       Impact factor: 4.379

3.  Generation of a Selective Small Molecule Inhibitor of the CBP/p300 Bromodomain for Leukemia Therapy.

Authors:  Sarah Picaud; Oleg Fedorov; Angeliki Thanasopoulou; Katharina Leonards; Katherine Jones; Julia Meier; Heidi Olzscha; Octovia Monteiro; Sarah Martin; Martin Philpott; Anthony Tumber; Panagis Filippakopoulos; Clarence Yapp; Christopher Wells; Ka Hing Che; Andrew Bannister; Samuel Robson; Umesh Kumar; Nigel Parr; Kevin Lee; Dave Lugo; Philip Jeffrey; Simon Taylor; Matteo L Vecellio; Chas Bountra; Paul E Brennan; Alison O'Mahony; Sharlene Velichko; Susanne Müller; Duncan Hay; Danette L Daniels; Marjeta Urh; Nicholas B La Thangue; Tony Kouzarides; Rab Prinjha; Jürg Schwaller; Stefan Knapp
Journal:  Cancer Res       Date:  2015-11-09       Impact factor: 12.701
  3 in total

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