| Literature DB >> 23436369 |
Riet Hilhorst1, Liesbeth Houkes, Monique Mommersteeg, Joyce Musch, Adriënne van den Berg, Rob Ruijtenbeek.
Abstract
Peptide microarray technology can be used to identify substrates for recombinant kinases, to measure kinase activity and changes thereof in cell lysates and lysates from fresh frozen (tumor) tissue. The effect of kinase inhibitors on the kinase activities in relevant tissues can be investigated as well. The method for performing experiments on dynamic peptide microarrays with real-time readout is described, as well as the influence of assay parameters and suggestions for optimization of experiments.Entities:
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Year: 2013 PMID： 23436369 DOI： 10.1007/978-1-62703-284-1_21
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745