| Literature DB >> 23436368 |
Ilona U Rafalska-Metcalf1, Susan M Janicki.
Abstract
Imaging molecularly defined regions of chromatin in single living cells during transcriptional activation has the potential to provide new insight into gene regulatory mechanisms. Here, we describe a method for isolating cell lines with multi-copy arrays of reporter transgenes, which can be used for real-time high-resolution imaging of transcriptional activation dynamics in single cells.Mesh：
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Year: 2013 PMID： 23436368 DOI： 10.1007/978-1-62703-284-1_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745