| Literature DB >> 23435258 |
Yao Zu1, Xiangjun Tong, Zhanxiang Wang, Da Liu, Ruochuan Pan, Zhe Li, Yingying Hu, Zhou Luo, Peng Huang, Qian Wu, Zuoyan Zhu, Bo Zhang, Shuo Lin.
Abstract
We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator-like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (th) locus, and we observed effective gene modification that was transmitted through the germ line. We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism.Entities:
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Year: 2013 PMID: 23435258 DOI: 10.1038/nmeth.2374
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547