Rinku D Umrani1, Kishore M Paknikar. 1. Centre for Nanobioscience, Agharkar Research Institute, G G Agarkar Road, Pune 411004, Maharashtra, India.
Abstract
AIM: The correlation of diabetes and an imbalance in zinc homeostasis makes zinc-based therapy an attractive proposition. In this study, zinc oxide nanoparticles were evaluated for antidiabetic effects and safety. MATERIALS & METHODS: Zinc oxide nanoparticles (1, 3 and 10 mg/kg) were tested for antidiabetic activity in streptozotocin-induced Type 1 and 2 diabetic rats. A single-dose pharmacokinetic study, cytotoxicity, hemolysis, acute and subacute toxicity tests, and mechanism-of-action studies were performed. RESULTS: Oral administration of zinc oxide nanoparticles resulted in significant antidiabetic effects--that is, improved glucose tolerance, higher serum insulin (70%), reduced blood glucose (29%), reduced nonesterified fatty acids (40%) and reduced triglycerides (48%). Nanoparticles were systemically absorbed resulting in elevated zinc levels in the liver, adipose tissue and pancreas. Increased insulin secretion and superoxide dismutase activity were also seen in rat insulinoma (RIN-5F) cells. Nanoparticles were safe up to a 300 mg/kg dose in rats. CONCLUSION: Zinc oxide nanoparticles are a promising antidiabetic agent warranting further studies.
AIM: The correlation of diabetes and an imbalance in zinc homeostasis makes zinc-based therapy an attractive proposition. In this study, zinc oxide nanoparticles were evaluated for antidiabetic effects and safety. MATERIALS & METHODS:Zinc oxide nanoparticles (1, 3 and 10 mg/kg) were tested for antidiabetic activity in streptozotocin-induced Type 1 and 2 diabeticrats. A single-dose pharmacokinetic study, cytotoxicity, hemolysis, acute and subacute toxicity tests, and mechanism-of-action studies were performed. RESULTS: Oral administration of zinc oxide nanoparticles resulted in significant antidiabetic effects--that is, improved glucose tolerance, higher serum insulin (70%), reduced blood glucose (29%), reduced nonesterified fatty acids (40%) and reduced triglycerides (48%). Nanoparticles were systemically absorbed resulting in elevated zinc levels in the liver, adipose tissue and pancreas. Increased insulin secretion and superoxide dismutase activity were also seen in ratinsulinoma (RIN-5F) cells. Nanoparticles were safe up to a 300 mg/kg dose in rats. CONCLUSION:Zinc oxide nanoparticles are a promising antidiabetic agent warranting further studies.
Authors: Norhan Elassy; Shady El-Dafrawy; Amira O Abd El-Azim; Om Ali Y El-Khawaga; Amr Negm Journal: IET Nanobiotechnol Date: 2020-10 Impact factor: 1.847