| Literature DB >> 23421517 |
Fa-Rong Li1, Yan-Yan Fu, Dan-Hong Jiang, Zhen Wu, Yang-Jing Zhou, Ling Guo, Zhong-Min Dong, Zhe-Zhi Wang.
Abstract
Multidrug resistance (MDR) has been a major problem in cancer chemotherapy. In this study, the aim was to explore the reversal effect and its potential mechanism of rosmarinic acid (RA) on SGC7901/Adr cells. 3-(4,5-Dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to investigate the reversal index of RA in SGC7901/Adr cell line. The intracellular accumulation of adriamycin, rhodamine123 (Rh123), and the expression of P-glycoprotein (P-gp) were assayed by flow cytometry. The influence of RA on the transcription of MDR1 gene was determined by reverse transcription-polymerase chain reaction. The results showed that RA could reverse the MDR of SGC7901/Adr cells, increase the intracellular accumulation of Adr and Rh123, and decrease the transcription of MDR1 gene and the expression of P-gp in SGC7901/Adr cells. These results indicated that RA was a potential multidrug resistance-reversing agent and warranted further investigations.Entities:
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Year: 2013 PMID： 23421517 DOI： 10.1080/10286020.2012.762910
Source DB: PubMed Journal: J Asian Nat Prod Res ISSN： 1028-6020 Impact factor: 1.569