Characterization of vitamin B₁₂compounds in the wild edible mushrooms black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius).

This study determined the vitamin B₁₂ content of six wild edible mushrooms which are consumed by European vegetarians. Zero or trace levels (0.01-0.09 µg/100 g dry weight) of vitamin B₁₂ were determined in porcini mushrooms (Boletus spp.), parasol mushrooms (Macrolepiota procera), oyster mushrooms (Pleurotus ostreatus), and black morels (Morchella conica). By contrast, black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius) mushrooms contained considerable levels (1.09-2.65 µg/100 g dry weight) of vitamin B₁₂. To determine whether C. cornucopioides or C. cibarius contained vitamin B₁₂ or other corrinoid compounds that are inactive in humans, we purified a corrinoid compound using an immunoaffinity column and identified it as vitamin B₁₂ based on LC/ESI-MS/MS chromatograms.