| Literature DB >> 23416147 |
Lovleen Saxena1, G Ravi Kumar, Shikha Saxena, Uttara Chaturvedi, Aditya Prasad Sahoo, Lakshya Veer Singh, Lakshman Santra, S K Palia, G S Desai, A K Tiwari.
Abstract
Apoptosis is programmed cell death that normally occurs during development and aging in multicellular animals. Apoptosis also occurs as a defense mechanism against disease or harmful external agents. It can be initiated by a variety of stimuli including viruses and viral proteins. Canine parvovirus type 2 (CPV-2) that causes acute disease in dogs has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. Though non structural protein 1 (NS1) of many parvoviruses has been found to be apoptotic, no report on the apoptotic potential of NS1 of CPV-2 (CPV-2.NS1) exists. In this study, we evaluated the apoptotic potential of CPV-2.NS1 in HeLa cells. CPV-2.NS1 has been found to induce apoptosis which was evident through characteristic DNA fragmentation, increase in hypodiploid cell count, phosphatidyl serine translocation and activation of caspase-3. Increase in caspase-3 activity and no change in p53 activity with time in CPV-2.NS1 expressing HeLa cells showed the induction of apoptosis to be caspase dependent and p53 independent.Entities:
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Year: 2013 PMID： 23416147 DOI： 10.1016/j.virusres.2013.01.020
Source DB: PubMed Journal: Virus Res ISSN： 0168-1702 Impact factor: 3.303