OBJECTIVE: To investigate the association between one-carbon metabolism and recurrent pregnancy loss (RPL). One-carbon metabolism is important for maintaining pregnancy, and the enzymes codified by these genes are relevant to this metabolic pathway. DESIGN: Case-control study. SETTING: An urban university-based hospital in South Korea. PATIENT(S): A cohort of 353 RPL patients (3.09 ± 1.65 pregnancy losses) and 226 control subjects. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Genotyping was assessed by polymerase chain reaction-restriction fragment length polymorphism assay. We examined polymorphisms in four genes: methionine synthase (MTR); methionine synthase reductase (MTRR); methylenetetrahydrofolate dehydrogenase 1 (MTHFD1); and thymidylate synthase (TS). RESULT(S): The MTR 2756AA polymorphism was associated with RPL. Gene-gene interaction analysis revealed that the frequency of the MTR 2756A-TS 6-bp allele combination was significantly higher in RPL. CONCLUSION(S): Based on these results, we propose that the MTR 2756AA genotype and MTR 2756A-TS 6-bp allele combination are possible predisposing factors for RPL development in Korean women.
OBJECTIVE: To investigate the association between one-carbon metabolism and recurrent pregnancy loss (RPL). One-carbon metabolism is important for maintaining pregnancy, and the enzymes codified by these genes are relevant to this metabolic pathway. DESIGN: Case-control study. SETTING: An urban university-based hospital in South Korea. PATIENT(S): A cohort of 353 RPL patients (3.09 ± 1.65 pregnancy losses) and 226 control subjects. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Genotyping was assessed by polymerase chain reaction-restriction fragment length polymorphism assay. We examined polymorphisms in four genes: methionine synthase (MTR); methionine synthase reductase (MTRR); methylenetetrahydrofolate dehydrogenase 1 (MTHFD1); and thymidylate synthase (TS). RESULT(S): The MTR 2756AA polymorphism was associated with RPL. Gene-gene interaction analysis revealed that the frequency of the MTR 2756A-TS 6-bp allele combination was significantly higher in RPL. CONCLUSION(S): Based on these results, we propose that the MTR 2756AA genotype and MTR 2756A-TS 6-bp allele combination are possible predisposing factors for RPL development in Korean women.
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