Literature DB >> 2341408

Purification and biochemical characterization of hepatocyte nuclear factor 2 involved in liver-specific transcription of the human alpha 1-antitrypsin gene.

V S Rangan1, G C Das.   

Abstract

The upstream region of the human alpha 1-antitrypsin gene binds liver-specific proteins. Two of these proteins, hepatocyte nuclear factors 1 and 2, are essential for liver-specific transcription of this gene. We report for the first time the purification of hepatocyte nuclear factor 2 from rat liver nuclei. This protein, purified to apparent homogeneity by DNA sequence-specific affinity chromatography, appeared on sodium dodecyl sulfate-polyacrylamide gels as a single polypeptide band of molecular mass of 68 kDa. The polypeptide after extraction from the gel matrix and subsequent renaturation bound specifically to the recognition sequences (-88 to -125) and protected the same region of the promoter against DNase I digestion.

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Year:  1990        PMID： 2341408

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  6 in total

1.  The transcription factor LF-A1 interacts with a bipartite recognition sequence in the promoter regions of several liver-specific genes.

Authors:  D P Ramji; M H Tadros; E M Hardon; R Cortese
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

2.  Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein.

Authors:  F Brunel; P M Alzari; P Ferrara; M M Zakin
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

3.  Interplay of an original combination of factors: C/EBP, NFY, HNF3, and HNF1 in the rat aldolase B gene promoter.

Authors:  M Raymondjean; A L Pichard; C Gregori; F Ginot; A Kahn
Journal:  Nucleic Acids Res       Date:  1991-11-25       Impact factor: 16.971

4.  Cis- and trans-acting elements required for constitutive and cytokine-regulated expression of the mouse complement C3 gene.

Authors:  N Kawamura; L Singer; R A Wetsel; H R Colten
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

5.  Constitutive and modulated expression of the human alpha 1 antitrypsin gene. Different transcriptional initiation sites used in three different cell types.

Authors:  W Hafeez; G Ciliberto; D H Perlmutter
Journal:  J Clin Invest       Date:  1992-04       Impact factor: 14.808

6.  Isolation and identification of cell-specific microRNAs targeting a messenger RNA using a biotinylated anti-sense oligonucleotide capture affinity technique.

Authors:  Tidi Hassan; Stephen G J Smith; Kevin Gaughan; Irene K Oglesby; Shane O'Neill; Noel G McElvaney; Catherine M Greene
Journal:  Nucleic Acids Res       Date:  2013-01-15       Impact factor: 16.971
  6 in total

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