Molecular characterization of a complete genome and 12 Nsp2 genes of PRRSV of southwestern China.

One genome and 12 Nsp2 genes of porcine reproductive and respiratory syndrome virus (PRRSV), isolated from southwestern China during 2009-2010, were sequenced and analyzed. The whole genome comparison analysis revealed that SCwhn09CD is a North American-type PRRSV with separate 30aa deletion in Nsp2 gene, and its sequence highly identical to highly pathogenic PRRSV (HP-PRRSV) outbreak in 2006 in almost half area of China. However, an extra 7aa deletion was detected in its Nsp2 coding region. Based on the phylogenetic analysis of Nsp2 gene, all the 12 viruses were grouped into North American-type PRRSV, but two in classical-PRRSV (C-PRRSV) subcluster without typical 30aa deletion and ten in HP-PRRSV subcluster with the signature deletion. In addition, many nucleotide substitutions were found in all 12 Nsp2 genes and extra three novel deletions were identified in three out of ten HP-PRRSV. Combining the above data, it may be concluded that: (1) HP-PRRSV and C-PRRSV co-existed in the local pig farms and the majority of the viruses is HR-PRRSV, and (2) the HR-PRRSV continues to undergo its genomic divergence although it does not outbreak in large-scale in recent years. Therefore, continuous surveillance on PRRSV is necessary for the controlling of the virus in this region.