Hong-Seop Kho1, Ji-Youn Chang, Yoon-Young Kim, Yongdae Kim. 1. Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Yunkeun-Dong 28, Chongro-Ku, Seoul 110-749, Republic of Korea. hkho@snu.ac.kr
Abstract
OBJECTIVE: Expression levels of MUC1 and TLR-2 were evaluated in burning mouth syndrome (BMS) patients and compared with those of controls and oral lichen planus (OLP) patients. The relationships between the expression levels of MUC1 and TLR-2 and levels of salivary pro-inflammatory cytokines were also investigated. DESIGN: Ten female BMS and ten female OLP patients were included. Ten female age-matched volunteers served as controls. RNA was isolated from stimulated whole saliva samples. Real-time PCR was used to quantify MUC1 and TLR-2 mRNA levels relative to β-actin and GAPDH mRNA levels. The clarified supernatants of saliva samples were used to measure IL-1β, IL-6, IL-8, and TNF-α levels. The level of blood contamination in saliva samples was also determined. RESULTS: There were significant increases in MUC1 transcripts in BMS patients compared with OLP patients (1.766-fold) as well as controls (1.840-fold). There was no significant difference in TLR-2 expression among the groups. The OLP patients showed significantly higher levels of IL-6 and blood contamination in saliva than other groups. The levels of MUC1 or TLR-2 expression did not correlate significantly with the levels of cytokines or blood contamination in saliva. CONCLUSIONS: MUC1 may play a role in the development and/or progression of BMS.
OBJECTIVE: Expression levels of MUC1 and TLR-2 were evaluated in burning mouth syndrome (BMS) patients and compared with those of controls and oral lichen planus (OLP) patients. The relationships between the expression levels of MUC1 and TLR-2 and levels of salivary pro-inflammatory cytokines were also investigated. DESIGN: Ten female BMS and ten female OLP patients were included. Ten female age-matched volunteers served as controls. RNA was isolated from stimulated whole saliva samples. Real-time PCR was used to quantify MUC1 and TLR-2 mRNA levels relative to β-actin and GAPDH mRNA levels. The clarified supernatants of saliva samples were used to measure IL-1β, IL-6, IL-8, and TNF-α levels. The level of blood contamination in saliva samples was also determined. RESULTS: There were significant increases in MUC1 transcripts in BMS patients compared with OLP patients (1.766-fold) as well as controls (1.840-fold). There was no significant difference in TLR-2 expression among the groups. The OLP patients showed significantly higher levels of IL-6 and blood contamination in saliva than other groups. The levels of MUC1 or TLR-2 expression did not correlate significantly with the levels of cytokines or blood contamination in saliva. CONCLUSIONS:MUC1 may play a role in the development and/or progression of BMS.
Authors: Natália Guimarães Barbosa; Amanda Katarinny Goes Gonzaga; Luzia Leiros de Sena Fernandes; Aldilane Gonçalves da Fonseca; Salomão Israel Monteiro Lourenço Queiroz; Telma Maria Araújo Moura Lemos; Éricka Janine Dantas da Silveira; Ana Miryam Costa de Medeiros Journal: Lasers Med Sci Date: 2018-03-03 Impact factor: 3.161