Literature DB >> 23410922

Expression and characterization of the nitrile reductase queF from E. coli.

Kathrin Moeller1, Giang-Son Nguyen, Frank Hollmann, Ulf Hanefeld.   

Abstract

The expression and characterization of a nitrile reductase from Escherichia coli K-12 (EcoNR), a newly discovered enzyme class, is described. This enzyme has a potential application for an alternative nitrile reduction pathway. The enzyme activity towards its natural substrate, preQ(0), was demonstrated and optimal working conditions were found to be at 37°C and at pH 7 with Tris buffer.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID: 23410922     DOI: 10.1016/j.enzmictec.2012.12.003

Source DB:  PubMed          Journal:  Enzyme Microb Technol        ISSN: 0141-0229            Impact factor:   3.493


  4 in total

1.  Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli.

Authors:  Jihye Jung; Bernd Nidetzky
Journal:  J Biol Chem       Date:  2018-01-16       Impact factor: 5.157

2.  Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide.

Authors:  Adeba Mohammad; Adriana Bon Ramos; Bobby W K Lee; Spencer W Cohen; Maryam K Kiani; Dirk Iwata-Reuyl; Boguslaw Stec; Manal A Swairjo
Journal:  Biomolecules       Date:  2017-03-16

Review 3.  Gut Glucosinolate Metabolism and Isothiocyanate Production.

Authors:  Arjan Narbad; John Trevor Rossiter
Journal:  Mol Nutr Food Res       Date:  2018-07-05       Impact factor: 5.914

Review 4.  Biocatalytic Reduction Reactions from a Chemist's Perspective.

Authors:  Frank Hollmann; Diederik J Opperman; Caroline E Paul
Journal:  Angew Chem Int Ed Engl       Date:  2020-11-03       Impact factor: 15.336

  4 in total

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