| Literature DB >> 23408130 |
Abstract
Bacteria within arthropods can be identified using culture-independent methods. This unit describes protocols for surface sterilization of arthropods, DNA extraction of whole bodies and tissues, touchdown PCR amplification using 16S rDNA general bacteria primers, and profiling the bacterial community using denaturing gradient gel electrophoresis.Entities:
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Year: 2013 PMID: 23408130 PMCID: PMC3625245 DOI: 10.1002/9780471729259.mc01e06s28
Source DB: PubMed Journal: Curr Protoc Microbiol