BACKGROUND: Emerging evidence has highlighted the pivotal role of the immune system in neurodegenerative diseases. This study investigated the impact of progressive neurodegeneration on the differentiation and development of hematopoietic stem cells in the peripheral blood of Parkinson's patients. METHODS: A colony-forming cell assay was established to study hematopoietic stem cells from venous blood of Parkinson's patients, and flow cytometry was used to analyze the expression of chemokine receptors on monocytes. RESULTS: We demonstrate that there is strong upregulation in the percentage of monocyte precursors in the peripheral blood of Parkinson's patients and asymptomatic high-risk individuals. We identify the receptor CCR2 as undergoing strong upregulation on the surface of classical monocytes in Parkinson's patients. CONCLUSIONS: The association between blood cell development and progressive cell death in the brain of Parkinson's patients should be further investigated as a potential dynamic biomarker and indicator of disease progression.
BACKGROUND: Emerging evidence has highlighted the pivotal role of the immune system in neurodegenerative diseases. This study investigated the impact of progressive neurodegeneration on the differentiation and development of hematopoietic stem cells in the peripheral blood of Parkinson'spatients. METHODS: A colony-forming cell assay was established to study hematopoietic stem cells from venous blood of Parkinson's patients, and flow cytometry was used to analyze the expression of chemokine receptors on monocytes. RESULTS: We demonstrate that there is strong upregulation in the percentage of monocyte precursors in the peripheral blood of Parkinson'spatients and asymptomatic high-risk individuals. We identify the receptor CCR2 as undergoing strong upregulation on the surface of classical monocytes in Parkinson'spatients. CONCLUSIONS: The association between blood cell development and progressive cell death in the brain of Parkinson'spatients should be further investigated as a potential dynamic biomarker and indicator of disease progression.
Authors: Holly Green; Xiaoqun Zhang; Katarina Tiklova; Nikolaos Volakakis; Lovisa Brodin; Louise Berg; Paul Greengard; Thomas Perlmann; Per Svenningsson Journal: Proc Natl Acad Sci U S A Date: 2017-01-30 Impact factor: 11.205
Authors: Hongwei Qin; Jessica A Buckley; Xinru Li; Yudong Liu; Thomas H Fox; Gordon P Meares; Hao Yu; Zhaoqi Yan; Ashley S Harms; Yufeng Li; David G Standaert; Etty N Benveniste Journal: J Neurosci Date: 2016-05-04 Impact factor: 6.167
Authors: Ashley S Harms; Aaron D Thome; Zhaoqi Yan; Aubrey M Schonhoff; Gregory P Williams; Xinru Li; Yudong Liu; Hongwei Qin; Etty N Benveniste; David G Standaert Journal: Exp Neurol Date: 2017-11-16 Impact factor: 5.330