INTRODUCTION: Peanut allergy is an increasingly serious disorder with a heterogeneous pattern of sensitization across different countries. In vitro diagnostic techniques may help in establishing these patterns. OBJECTIVES: To analyze the usefulness of determining specific immunoglobulin E (sIgE) with the ImmunoCAP fluorescence enzyme immunoassay (FEIA), the ImmunoCAP ISAC CRD103 microarray (ISAC), and the basophil activation test (BAT) in the molecular diagnosis of peanut allergy. METHODS: In 26 peanut-allergic patients, sIgE antibodies against allergic components were measured with FEIA, ISAC, and BAT. RESULTS: The major peanut component in our population wasAra h 9.The detection of sIgE toAra h 9 using FEIA and BAT with this allergen yielded a sensitivity of 92% and 88% and a specificity of 95% and 100%, respectively. Overall diagnosis of peanut allergy by ISAC showed a sensitivity of 11% but a specificity of 95% since Ara h 9 was not present in the microarray version used. There was diagnostic agreement between the 3 techniques for the peanut allergens studied. CONCLUSIONS: The determination of sIgE to Ara h 9 using FEIA and BAT offers high sensitivity and specificity in the diagnosis of peanut allergy in the Spanish population. The CRD103 version of ISAC is not of value in our region as it does not include the most common allergen, Ara h 9.
INTRODUCTION:Peanutallergy is an increasingly serious disorder with a heterogeneous pattern of sensitization across different countries. In vitro diagnostic techniques may help in establishing these patterns. OBJECTIVES: To analyze the usefulness of determining specific immunoglobulin E (sIgE) with the ImmunoCAP fluorescence enzyme immunoassay (FEIA), the ImmunoCAP ISAC CRD103 microarray (ISAC), and the basophil activation test (BAT) in the molecular diagnosis of peanutallergy. METHODS: In 26 peanut-allergicpatients, sIgE antibodies against allergic components were measured with FEIA, ISAC, and BAT. RESULTS: The major peanut component in our population wasAra h 9.The detection of sIgE toAra h 9 using FEIA and BAT with this allergen yielded a sensitivity of 92% and 88% and a specificity of 95% and 100%, respectively. Overall diagnosis of peanutallergy by ISAC showed a sensitivity of 11% but a specificity of 95% since Ara h 9 was not present in the microarray version used. There was diagnostic agreement between the 3 techniques for the peanut allergens studied. CONCLUSIONS: The determination of sIgE to Ara h 9 using FEIA and BAT offers high sensitivity and specificity in the diagnosis of peanutallergy in the Spanish population. The CRD103 version of ISAC is not of value in our region as it does not include the most common allergen, Ara h 9.
Authors: Lucy Duan; Alper Celik; Jennifer A Hoang; Klara Schmidthaler; Delvin So; Xiaojun Yin; Christina M Ditlof; Marta Ponce; Julia E M Upton; Jean-Soo Lee; Lisa Hung; Heimo Breiteneder; Chiara Palladino; Adelle R Atkinson; Vy H D Kim; Alireza Berenjy; Maria Asper; David Hummel; Samantha Wong; Mara Alexanian-Farr; Ahuva Magder; Sharon R Chinthrajah; Kaori Mukai; Mindy Tsai; Kari Nadeau; Stephen J Galli; Arun K Ramani; Zsolt Szepfalusi; Thomas Eiwegger Journal: Allergy Date: 2020-12-29 Impact factor: 14.710
Authors: Alexandra F Santos; Abdel Douiri; Natalia Bécares; Shih-Ying Wu; Alick Stephens; Suzana Radulovic; Susan M H Chan; Adam T Fox; George Du Toit; Victor Turcanu; Gideon Lack Journal: J Allergy Clin Immunol Date: 2014-07-25 Impact factor: 10.793
Authors: Christian Lupinek; Eva Wollmann; Alexandra Baar; Srinita Banerjee; Heimo Breiteneder; Barbara M Broecker; Merima Bublin; Mirela Curin; Sabine Flicker; Tetiana Garmatiuk; Heidrun Hochwallner; Irene Mittermann; Sandra Pahr; Yvonne Resch; Kenneth H Roux; Bharani Srinivasan; Sebastian Stentzel; Susanne Vrtala; Leanna N Willison; Magnus Wickman; Karin C Lødrup-Carlsen; Josep Maria Antó; Jean Bousquet; Claus Bachert; Daniel Ebner; Thomas Schlederer; Christian Harwanegg; Rudolf Valenta Journal: Methods Date: 2013-10-22 Impact factor: 3.608