Literature DB >> 23397548

Simultaneous demonstration of gelatinolytic activity, morphology, and immunohistochemical reaction using zymography film.

Naoki Kanomata1, Takahiro Hasebe, Takuya Moriya, Atsushi Ochiai.   

Abstract

In situ zymography has been used to assess gelatinolytic activity, which is mainly due to matrix metalloproteinases (MMPs) in cancer tissues. MMPs play an important role in cancer invasion and metastasis. Film in situ zymography (FIZ) enables the in situ evaluation of gelatinolytic activity with high reproducibility. In this article, we report a study of FIZ, in a case of breast cancer with an invasive carcinoma component showing clear gelatinolytic activity, and in a non-invasive carcinoma component showing little gelatinolytic activity. Immunohistochemistry on FIZ was also performed. The simultaneous detection of gelatinolytic activity and immunohistochemical reaction was established in a single film. Immunohistochemistry on FIZ may have good potential for the investigation of cancer microenvironment.

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Year:  2013        PMID： 23397548     DOI： 10.1007/s00795-013-0021-4

Source DB:  PubMed          Journal:  Med Mol Morphol        ISSN： 1860-1499            Impact factor:   2.309


  25 in total

1.  Matrix metalloproteinase-9 in myeloid cells: implications for allergic inflammation.

Authors:  M Hayashi; R R Schellenberg; S Tsang; C R Roberts
Journal:  Int Arch Allergy Immunol       Date:  1999 Feb-Apr       Impact factor: 2.749

2.  In situ gelatinolytic activity correlates with tumor progression and prognosis in patients with bladder cancer.

Authors:  Koji Kawamura; Naoto Kamiya; Takahito Suyama; Masaki Shimbo; Nobuyuki Oosumi; Hiroyoshi Suzuki; Takeshi Ueda; Toyofusa Tobe; Tatsuo Igarashi; Haruo Ito; Hiroshi Ishikura
Journal:  J Urol       Date:  2004-10       Impact factor: 7.450

3.  Inflammation in atherosclerosis: visualizing matrix metalloproteinase action in macrophages in vivo.

Authors:  Jun-o Deguchi; Masanori Aikawa; Ching-Hsuan Tung; Elena Aikawa; Dong-Eog Kim; Vasilis Ntziachristos; Ralph Weissleder; Peter Libby
Journal:  Circulation       Date:  2006-06-26       Impact factor: 29.690

4.  Matrix metalloproteinases promote inflammation and fibrosis in asbestos-induced lung injury in mice.

Authors:  Roderick J Tan; Cheryl L Fattman; Laura M Niehouse; Jacob M Tobolewski; Lana E Hanford; Qinglang Li; Federico A Monzon; William C Parks; Tim D Oury
Journal:  Am J Respir Cell Mol Biol       Date:  2006-03-30       Impact factor: 6.914

5.  Inhibition of gelatinolytic activity in tumor tissues by synthetic matrix metalloproteinase inhibitor: application of film in situ zymography.

Authors:  M Ikeda; R Maekawa; H Tanaka; M Matsumoto; Y Takeda; Y Tamura; R Nemori; T Yoshioka
Journal:  Clin Cancer Res       Date:  2000-08       Impact factor: 12.531

6.  Evaluation of matrix metalloproteinase-2 (MMP-2) activity with film in situ zymography for improved cytological diagnosis of breast tumors.

Authors:  Akira Fujiwara; Eri Shibata; Hitomi Terashima; Akemi Shishido; Junko Nishiki; Kyotaro Yoshida; Keisuke Miyauchi; Ayako Madachi; Nariaki Matsuura
Journal:  Breast Cancer       Date:  2006       Impact factor: 4.239

7.  Expression and localization of mRNAs for matrix metalloproteinases and their inhibitors in mixed bronchioloalveolar carcinomas with invasive components.

Authors:  Naoki Kanomata; Rie Nakahara; Tatsuya Oda; Yasuyuki Aoyagi; Genichiro Ishii; Tomoyuki Yokose; Takahiro Hasebe; Kanji Nagai; Hiroshi Yokozaki; Atsushi Ochiai
Journal:  Mod Pathol       Date:  2005-06       Impact factor: 7.842

8.  Expression and tissue localization of membrane-type 1, 2, and 3 matrix metalloproteinases in human astrocytic tumors.

Authors:  M Nakada; H Nakamura; E Ikeda; N Fujimoto; J Yamashita; H Sato; M Seiki; Y Okada
Journal:  Am J Pathol       Date:  1999-02       Impact factor: 4.307

9.  Enhanced production and activation of progelatinase A mediated by membrane-type 1 matrix metalloproteinase in human papillary thyroid carcinomas.

Authors:  H Nakamura; H Ueno; K Yamashita; T Shimada; E Yamamoto; M Noguchi; N Fujimoto; H Sato; M Seiki; Y Okada
Journal:  Cancer Res       Date:  1999-01-15       Impact factor: 12.701

10.  Early acquisition of gelatinolytic activity in carcinogenesis of the uterine cervix.

Authors:  Rie Minami; Hajime Tsunoda; Tatsuo Iijima; Hiroyuki Yoshikawa; Ryoichi Nemori; Masayuki Noguchi
Journal:  Mod Pathol       Date:  2003-11       Impact factor: 7.842
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