BACKGROUND: Beta-human papillomavirus (betaPV) may play a role in the development of cutaneous squamous cell carcinoma (SCC). However betaPV is highly prevalent, and it may only be people with a higher viral load who have increased risk of SCCs. We therefore examined the association between betaPV load and SCCs. METHODS: We recruited 448 immunocompetent cases with SCCs and 464 controls from Italy and Australia and 497 immunosuppressed organ transplant recipients (OTR; 179 cases and 318 controls) from Europe. We used reverse hybridization to genotype 25 betaPV types in eyebrow hair follicles and determined the viral load for eight selected types using quantitative PCR. We used logistic regression to assess associations between type-specific and cumulative viral load and SCCs. RESULTS: Australian and OTR participants in the highest cumulative load tertile were at significantly higher risk of SCCs than those in the lowest tertile. Those with more than four betaPV types in the high load tertile were at approximately three-fold increased risk of SCCs. In Australia, HPV23 and 36 loads were significantly associated with SCCs, with borderline associations for HPV5 and 38. In OTR, HPV8 and 38 loads were significantly associated and HPV20 and 36 were borderline. We found little evidence for an association between load and SCCs in Italy. CONCLUSIONS: High viral load may be associated with risk of cutaneous SCCs, with total load seemingly more important than the load of any specific type. IMPACT: Our findings lend weight to the hypothesis that HPV plays a role in skin carcinogenesis.
BACKGROUND: Beta-human papillomavirus (betaPV) may play a role in the development of cutaneous squamous cell carcinoma (SCC). However betaPV is highly prevalent, and it may only be people with a higher viral load who have increased risk of SCCs. We therefore examined the association between betaPV load and SCCs. METHODS: We recruited 448 immunocompetent cases with SCCs and 464 controls from Italy and Australia and 497 immunosuppressed organ transplant recipients (OTR; 179 cases and 318 controls) from Europe. We used reverse hybridization to genotype 25 betaPV types in eyebrow hair follicles and determined the viral load for eight selected types using quantitative PCR. We used logistic regression to assess associations between type-specific and cumulative viral load and SCCs. RESULTS: Australian and OTR participants in the highest cumulative load tertile were at significantly higher risk of SCCs than those in the lowest tertile. Those with more than four betaPV types in the high load tertile were at approximately three-fold increased risk of SCCs. In Australia, HPV23 and 36 loads were significantly associated with SCCs, with borderline associations for HPV5 and 38. In OTR, HPV8 and 38 loads were significantly associated and HPV20 and 36 were borderline. We found little evidence for an association between load and SCCs in Italy. CONCLUSIONS: High viral load may be associated with risk of cutaneous SCCs, with total load seemingly more important than the load of any specific type. IMPACT: Our findings lend weight to the hypothesis that HPV plays a role in skin carcinogenesis.
Authors: Dana E Rollison; Michael J Schell; Neil A Fenske; Basil Cherpelis; Jane L Messina; Anna R Giuliano; Pearlie K Epling-Burnette; Shalaka S Hampras; Rossybelle P Amorrortu; Juliana Balliu; Laxmi Vijayan; Syeda Mahrukh Hussnain Naqvi; Yayi Zhao; Kaustubh Parab; Sandrine McKay-Chopin; Tarik Gheit; Massimo Tommasino Journal: J Infect Dis Date: 2019-02-15 Impact factor: 5.226
Authors: Roel E Genders; Hadi Mazlom; Angelika Michel; Elsemieke I Plasmeijer; Koen D Quint; Michael Pawlita; Els van der Meijden; Tim Waterboer; Hans de Fijter; Frans H Claas; Ron Wolterbeek; Mariet C W Feltkamp; Jan Nico Bouwes Bavinck Journal: J Invest Dermatol Date: 2014-10-27 Impact factor: 8.551
Authors: Gian Paolo Marcuzzi; Sabine Awerkiew; Martin Hufbauer; Lysann Schädlich; Lutz Gissmann; Sabine Eming; Herbert Pfister Journal: Med Microbiol Immunol Date: 2014-01-21 Impact factor: 3.402
Authors: Ilir Agalliu; Susan Gapstur; Zigui Chen; Tao Wang; Rebecca L Anderson; Lauren Teras; Aimée R Kreimer; Richard B Hayes; Neal D Freedman; Robert D Burk Journal: JAMA Oncol Date: 2016-05-01 Impact factor: 31.777
Authors: Dana E Rollison; Rossybelle P Amorrortu; Yayi Zhao; Jane L Messina; Michael J Schell; Neil A Fenske; Basil S Cherpelis; Anna R Giuliano; Vernon K Sondak; Michael Pawlita; Sandrine McKay-Chopin; Tarik Gheit; Tim Waterboer; Massimo Tommasino Journal: Cancer Res Date: 2021-07-15 Impact factor: 12.701