| Literature DB >> 23395636 |
Lieven Haenebalcke1, Steven Goossens, Pieterjan Dierickx, Sonia Bartunkova, Jinke D'Hont, Katharina Haigh, Tino Hochepied, Dagmar Wirth, Andras Nagy, Jody J Haigh.
Abstract
Control of cellular (de)differentiation in a temporal, cell-specific, and exchangeable manner is of paramount importance in the field of reprogramming. Here, we have generated and characterized a mouse strain that allows iPSC generation through the Cre/loxP conditional and doxycycline/rtTA-controlled inducible expression of the OSKM reprogramming factors entirely from within the ROSA26 locus. After reprogramming, these factors can be replaced by genes of interest-for example, to enhance lineage-directed differentiation-with the use of a trap-coupled RMCE reaction. We show that, similar to ESCs, Dox-controlled expression of the cardiac transcriptional regulator Mesp1 together with Wnt inhibition enhances the generation of functional cardiomyocytes upon in vitro differentiation of such RMCE-retargeted iPSCs. This ROSA26-iPSC mouse model is therefore an excellent tool for studying both cellular reprogramming and lineage-directed differentiation factors from the same locus and will greatly facilitate the identification and ease of functional characterization of the genetic/epigenetic determinants involved in these complex processes.Entities:
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Year: 2013 PMID： 23395636 DOI： 10.1016/j.celrep.2013.01.016
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423