PURPOSE: To investigate the ability of a supercharge end-to-side (SETS) nerve transfer to augment the effect of regenerating native axons in an incomplete rodent sciatic nerve injury model. METHODS: Fifty-four Lewis rats were randomized to 3 groups. The first group was an incomplete recovery model (IRM) of the tibial nerve complemented with an SETS transfer from the peroneal nerve (SETS-IRM). The IRM consisted of tibial nerve transection and immediate repair using a 10-mm fresh tibial isograft to provide some, but incomplete, nerve recovery. The 2 control groups were IRM alone and SETS alone. Nerve histomorphometry, electron microscopy, retrograde labeling, and muscle force testing were performed. RESULTS: Histomorphometry of the distal tibial nerve showed significantly increased myelinated axonal counts in the SETS-IRM group compared with the IRM and SETS groups at 5 and 8 weeks. Retrograde labeling at 8 weeks confirmed increased motoneuron counts in the SETS-IRM group. Functional recovery at 8 weeks showed a significant increase in muscle-specific force in the SETS-IRM group compared with the IRM group. CONCLUSIONS: An SETS transfer enhanced recovery from an incomplete nerve injury as determined by histomorphometry, motoneuron labeling within the spinal cord, and muscle force measurements. CLINICAL RELEVANCE: An SETS distal nerve transfer may be useful in nerve injuries with incomplete regeneration such as proximal Sunderland II- or III-degree injuries, in which long regeneration distance yields prolonged time to muscle reinnervation and suboptimal functional recovery.
PURPOSE: To investigate the ability of a supercharge end-to-side (SETS) nerve transfer to augment the effect of regenerating native axons in an incomplete rodent sciatic nerve injury model. METHODS: Fifty-four Lewis rats were randomized to 3 groups. The first group was an incomplete recovery model (IRM) of the tibial nerve complemented with an SETS transfer from the peroneal nerve (SETS-IRM). The IRM consisted of tibial nerve transection and immediate repair using a 10-mm fresh tibial isograft to provide some, but incomplete, nerve recovery. The 2 control groups were IRM alone and SETS alone. Nerve histomorphometry, electron microscopy, retrograde labeling, and muscle force testing were performed. RESULTS: Histomorphometry of the distal tibial nerve showed significantly increased myelinated axonal counts in the SETS-IRM group compared with the IRM and SETS groups at 5 and 8 weeks. Retrograde labeling at 8 weeks confirmed increased motoneuron counts in the SETS-IRM group. Functional recovery at 8 weeks showed a significant increase in muscle-specific force in the SETS-IRM group compared with the IRM group. CONCLUSIONS: An SETS transfer enhanced recovery from an incomplete nerve injury as determined by histomorphometry, motoneuron labeling within the spinal cord, and muscle force measurements. CLINICAL RELEVANCE: An SETS distal nerve transfer may be useful in nerve injuries with incomplete regeneration such as proximal Sunderland II- or III-degree injuries, in which long regeneration distance yields prolonged time to muscle reinnervation and suboptimal functional recovery.
Authors: Amy M Moore; Gregory H Borschel; Katherine B Santosa; Eric R Flagg; Alice Y Tong; Rahul Kasukurthi; Piyaraj Newton; Ying Yan; Daniel A Hunter; Philip J Johnson; Susan E Mackinnon Journal: J Neurosci Methods Date: 2011-10-20 Impact factor: 2.390
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Authors: Rahul Kasukurthi; Michael J Brenner; Amy M Moore; Arash Moradzadeh; Wilson Z Ray; Katherine B Santosa; Susan E Mackinnon; Daniel A Hunter Journal: J Neurosci Methods Date: 2009-08-31 Impact factor: 2.390
Authors: D C Riley; G D Bittner; M Mikesh; N L Cardwell; A C Pollins; C L Ghergherehchi; S R Bhupanapadu Sunkesula; T N Ha; B T D Hall; A D Poon; M Pyarali; R B Boyer; A T Mazal; N Munoz; R C Trevino; T Schallert; W P Thayer Journal: J Neurosci Res Date: 2014-11-25 Impact factor: 4.164
Authors: G D Bittner; D R Sengelaub; R C Trevino; J D Peduzzi; M Mikesh; C L Ghergherehchi; T Schallert; W P Thayer Journal: J Neurosci Res Date: 2015-11-03 Impact factor: 4.164