Genetic diversity and species pattern of Trichoderma and Hypocrea in Manipur using in silico analysis.

We investigated the occurrence and genetic diversity of Trichoderma and Hypocrea in Manipur which lies in the Indo-Burma biodiversity hot spot region. 65 Trichoderma isolates were identified at species level by morphological as well as sequence based analysis of the internal transcribed spacer region 1 and 4. Altogether 22 different species of Trichoderma and Hypocrea were found, of which Trichoderma harzianum represent the dominant species. Phylogenetic analysis reveals a clear cut distinction of strains isolated from various collection sites which further hints the need for detail study of Trichoderma on molecular level.