| Literature DB >> 23386338 |
Yuqing Chang1, Bo Yang, Amanda Weyers, Robert J Linhardt.
Abstract
Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.Entities:
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Year: 2013 PMID： 23386338 PMCID： PMC3875140 DOI： 10.1007/978-1-62703-296-4_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745