| Literature DB >> 23386296 |
Bharti Sharma1, Elena M Kramer.
Abstract
Aquilegia Origami is an emerging model system for ecology and evolution, which has numerous genetic and genomic tools. Virus-induced gene silencing (VIGS) has been established as an effective approach to study gene function in Aquilegia. In the current protocol, we demonstrate VIGS using Agrobacterium strain GV3101 carrying tobacco rattle virus (TRV)-based constructs to infect Aquilegia coerulea "Origami" plants via vacuum infiltration.Entities:
Mesh:
Year: 2013 PMID: 23386296 DOI: 10.1007/978-1-62703-278-0_6
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745