| Literature DB >> 23381856 |
Romeo Papazyan1, Sean D Taverna.
Abstract
High-performance liquid chromatography (HPLC) is extremely useful for the study of proteins and the characterization of their posttranslational modifications. Here we describe a method that utilizes cation-exchange HPLC to separate multiply acetylated histone H3 species on the basis of their charge and hydrophilicity. This high-resolution method allows for the separation of histone H3 species that differ by as few as one acetyl group, and is compatible with subsequent analysis by a variety of techniques, including mass spectrometry and western blotting.Entities:
Mesh：
Substances：
Year: 2013 PMID： 23381856 PMCID： PMC3779653 DOI： 10.1007/978-1-62703-305-3_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745