Aude Andouche1, Yann Bassaglia, Sébastien Baratte, Laure Bonnaud. 1. Muséum National d'Histoire Naturelle MNHN, DMPA, UMR Biologie des Organismes et Ecosystèmes Aquatiques BOREA, MNHN CNRS 7208, IRD 207, UPMC, 75005 Paris, France. andouche@mnhn.fr
Abstract
BACKGROUND: In the cuttlefish Sepia officinalis, iridescence is known to play a role in patterning and communication. In iridophores, iridosomes are composed of reflectins, a protein family, which show great diversity in all cephalopod species. Iridosomes are established before hatching, but very little is known about how these cells are established, their distribution in embryos, or the contribution of each reflectin gene to iridosome structures. RESULTS: Six reflectin genes are expressed during the development of iridosomes in Sepia officinalis. We show that they are expressed in numerous parts of the body before hatching. Evidence of the colocalization of two different genes of reflectin was found. Curiously, reflectin mRNA expression was no longer detectable at the time of hatchling, while reflectin proteins were present and gave rise to visible iridescence. CONCLUSION: These data suggest that several different forms of reflectins are simultaneously used to produce iridescence in S. officinalis and that mRNA production and translation are decoupled in time during iridosome development.
BACKGROUND: In the cuttlefish Sepia officinalis, iridescence is known to play a role in patterning and communication. In iridophores, iridosomes are composed of reflectins, a protein family, which show great diversity in all cephalopod species. Iridosomes are established before hatching, but very little is known about how these cells are established, their distribution in embryos, or the contribution of each reflectin gene to iridosome structures. RESULTS: Six reflectin genes are expressed during the development of iridosomes in Sepia officinalis. We show that they are expressed in numerous parts of the body before hatching. Evidence of the colocalization of two different genes of reflectin was found. Curiously, reflectin mRNA expression was no longer detectable at the time of hatchling, while reflectin proteins were present and gave rise to visible iridescence. CONCLUSION: These data suggest that several different forms of reflectins are simultaneously used to produce iridescence in S. officinalis and that mRNA production and translation are decoupled in time during iridosome development.
Authors: Leila F Deravi; Andrew P Magyar; Sean P Sheehy; George R R Bell; Lydia M Mäthger; Stephen L Senft; Trevor J Wardill; William S Lane; Alan M Kuzirian; Roger T Hanlon; Evelyn L Hu; Kevin Kit Parker Journal: J R Soc Interface Date: 2014-01-29 Impact factor: 4.118
Authors: Long Phan; Rylan Kautz; Janahan Arulmoli; Iris H Kim; Dai Trang T Le; Michael A Shenk; Medha M Pathak; Lisa A Flanagan; Francesco Tombola; Alon A Gorodetsky Journal: ACS Appl Mater Interfaces Date: 2015-12-24 Impact factor: 9.229