| Literature DB >> 2337596 |
R Rehfuss1, J Goodisman, J C Dabrowiak.
Abstract
The theory for measuring ligand binding constants from footprinting autoradiographic data associated with a single binding site is derived. If the ligand and DNA cleavage agent compete for a common site, the spot intensities are not proportional to the amount of DNA not blocked by ligand. The analysis of a single site is experimentally illustrated by using results for the anticancer drug actinomycin D interacting with the duplex d(TAGCGCTA)2 as probed with the hydrolytic enzyme DNase I.Entities:
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Year: 1990 PMID： 2337596 DOI： 10.1021/bi00455a027
Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162